ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Conventional electrophoretic methods of assessing the purity of a protein are both expensive and laborious. The method of zonal isoelectric precipitation electrophoresis involves precipitation of the protein under investigation at its isoelectric point after application onto cellulose acetate membranes. Contaminating proteins will be mobile and are easily visualized by conventional staining methods. Application of zonal isoelectric precipitation electrophoresis to the storage proteins of Vicia faba seeds (legumin and vicilin) has proved valuable in this laboratory, and, by adjustment of the buffering system, can be made to work for other protein mixtures.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150040415
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