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  • 1
    Electronic Resource
    Electronic Resource
    Further evidence of a cybridization requirement for plant regeneration from citrus leaf protoplasts following somatic fusion (1996)
    Springer
    Plant cell reports 15 (1996), S. 672-676 
    Details
    ISSN: 1432-203X
    Keywords: Abbreviations: cp-chloroplast; GOT-glutamate-oxaloacetate transaminase; IDH-isocitrate dehydrogenase; mt-mitochondria; PEG-polyethylene glycol; PGI-phosphoglucose isomerase; PGM-phosphoglucomutase; RFLP-restriction fragment length polymorphism ; Keywords: chloroplast, mitochondria, somatic embryogenesis, tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary. Somatic hybridization experiments in Citrus that involve the fusion of protoplasts of one parent isolated from either nucellus-derived embryogenic callus or suspension cultures with leaf-derived protoplasts of a second parent, often result in the regeneration of diploid plants that phenotypically resemble the leaf parent. In this study, plants of this type regenerated following somatic fusions of the following three parental combinations were analyzed to determine their genetic origin (nuclear and organelle): (embryogenic parent listed first, leaf parent second) (1) calamondin (C. microcarpa Bunge) + 'Keen' sour orange (C. aurantium (L.), (2) Cleopatra mandarin (C. reticulata Blanco) + sour orange, and (3) 'Valencia' sweet orange (C sinensis (L.) Osbeck) + 'Femminello' lemon (C. limon (L.) Burm. f.). Isozyme analyses of PGI, PGM, GOT, and IDH zymograms of putative cybrid plants, along with RFLP analyses using a nuclear genome-specific probe showed that these plants contained the nucleus of the leaf parent. RFLP analyses using mtDNA-specific probes showed that these plants contained the mitochondrial genome of the embryogenic callus donor, thereby confirming cybridization. RFLP analyses using cpDNA-specific probes revealed that the cybrid plants contained the chloroplast genome of either one or the other parent. These results support previous reports indicating that acquisition of the mitochondria of embryogenic protoplasts by leaf protoplasts in a prerequisite for recovering plants with the leaf parent phenotype via somatic embryogenesis following somatic fusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050096
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  • 2
    Electronic Resource
    Electronic Resource
    Further evidence of a cybridization requirement for plant regeneration from citrus leaf protoplasts following somatic fusion (1996)
    Springer
    Plant cell reports 15 (1996), S. 672-676 
    Details
    ISSN: 1432-203X
    Keywords: chloroplast ; mitochondria ; somatic embryogenesis ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Somatic hybridization experiments in Citrus that involve the fusion of protoplasts of one parent isolated from either nucellus-derived embryogenic callus or suspension cultures with leaf-derived protoplasts of a second parent, often result in the regeneration of diploid plants that phenotypically resemble the leaf parent. In this study, plants of this type regenerated following somatic fusions of the following three parental combinations were analyzed to determine their genetic origin (nuclear and organelle): (embryogenic parent listed first, leaf parent second) (1) calamondin (C. microcarpa Bunge) + ‘Keen’ sour orange (C. aurantium L.), (2) Cleopatra mandarin (C. reticulata Blanco) + sour orange, and (3) ‘Valencia’ sweet orange (C. sinensis (L.) Osbeck) + ‘Femminello’ lemon (C. limon (L.) Burm. f.). Isozyme analyses of PGI, PGM, GOT, and IDH zymograms of putative cybrid plants, along with RFLP analyses using a nuclear genome-specific probe showed that these plants contained the nucleus of the leaf parent. RFLP analyses using mtDNA-specific probes showed that these plants contained the mitochondrial genome of the embryogenic callus donor, thereby confirming cybridization. RFLP analyses using cpDNA-specific probes revealed that the cybrid plants contained the chloroplast genome of either one or the other parent. These results support previous reports indicating that acquisition of the mitochondria of embryogenic protoplasts by leaf protoplasts is a prerequisite for recovering plants with the leaf parent phenotype via somatic embryogenesis following somatic fusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231922
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  • 3
    Electronic Resource
    Electronic Resource
    Somatic hybrid plants from sexually incompatible woody species: Citrus reticulata and Citropsis gilletiana (1990)
    Springer
    Plant cell reports 8 (1990), S. 656-659 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Allotetraploid intergeneric somatic hybrid plants between Citrus reticulata Blanco cv. Cleopatra mandarin and Citropsis gilletiana Swing. & M. Kell. (common name Gillet's cherry orange) were regenerated following protoplast fusion. Cleopatra protoplasts were isolated from an ovule-derived embryogenic suspension culture and fused chemically with leaf-derived protoplasts of Citropsis gilletiana. Cleopatra mandarin and somatic hybrid plants were regenerated via somatic embryogenesis. Hybrid plant identification was based on differential leaf morphology, root-tip cell chromosome number, and electrophoretic analyses of phosphoglucose mutase (PGM) and phosphohexose isomerase (PHI) isozyme banding patterns. This is the first somatic hybrid within the Rutaceae reported that does not have Citrus sinensis (sweet orange) as a parent, and the first produced with a commercially important citrus rootstock and a complementary but sexually incompatible, related species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269986
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