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  • 1
    Publication Date: 2011-01-10
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 2
    Publication Date: 2011-01-26
    Description: WNK [with no lysine (K)] protein kinases are found in all sequenced multicellular and many unicellular organisms. WNKs influence ion balance. Two WNK family members are associated with a single gene form of hypertension. RNA interference screens have implicated WNKs in survival and growth, and WNK1 is essential for viability of mice. We found that the majority of WNK1 is localized on cytoplasmic puncta in resting cells. During cell division, WNK1 localizes to mitotic spindles. Therefore, we analyzed mitotic phenotypes in WNK1 knockdown cells. A large percentage of WNK1 knockdown cells fail to complete cell division, displaying defects in mitotic spindles and also in abscission and cell survival. One of the best-characterized WNK1 targets is the protein kinase OSR1 (oxidative stress responsive 1). OSR1 regulates ion cotransporters, is activated in response to osmotic stress by WNK family members, and is largely associated with WNK1. In resting cells, the majority of OSR1, like WNK1, is on cytoplasmic puncta. OSR1 is also in nuclei. In contrast to WNK1, however, OSR1 does not concentrate around spindles during mitosis and does not show a WNK1-like localization pattern in mitotic cells. Knockdown of OSR1 has only a modest effect on cell survival and does not lead to spindle defects. We conclude that decreased cell survival associated with loss of WNK1 is attributable to defects in chromosome segregation and abscission and is independent of the effector kinase OSR1.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 3
    Publication Date: 1983-12-01
    Description: The main experimental results of the study of periodic turbulent pipe flow have been described in Part 1 of this report. In this second part, these experimental data are examined in greater detail to understand the effect of imposed oscillation on the flow structure, at moderate to large oscillation frequencies. Data on phase and amplitude and energy spectrum are used to study the effect of the imposed oscillation on the turbulence structure at these interactive frequencies of oscillation. Additional experiments which were performed to study the effect of oscillation frequency on the flow structure are also reported. Based on the present observations as well as on the data from other sources, it is inferred that turbulent shear flows respond very differently from laminar shear flows to imposed unsteadiness. A turbulent Stokes number relevant for characterizing the unsteady turbulent shear flows is identified and used to classify such flows. © 1983, Cambridge University Press. All rights reserved.
    Print ISSN: 0022-1120
    Electronic ISSN: 1469-7645
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
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  • 4
    Publication Date: 1983-12-01
    Description: The present paper is the first part of a two part report on a detailed investigation of periodic turbulent pipe flow. In this investigation, experimental data on instantaneous velocity and wall shear stress were obtained at a mean Reynolds number of 50000 in a fully developed turbulent pipe flow in which the volumetric flow rate was varied sinusoidally with time around the mean. Two oscillation frequencies at significant levels of flow modulation were studied in detail. The higher of these frequencies was of the order of the turbulent bursting frequency in the flow, and the other can be regarded as an intermediate frequency at which the flow still departed significantly from quasi steady behaviour. While a few similar experiments have been reported in the recent literature, the present study stands out from the others in respect of the flow regimes investigated, the magnitude of flow modulation, the detailed nature of the measurements and most importantly the identification of a relevant parameter to characterize unsteady shear flows. The present paper contains the main experimental results and comparisons of these results with the results of a numerical calculation procedure which employs a well known quasi steady turbulence closure model. The experimental data are used to study the manner in which the time-mean, the ensemble averaged and the random flow properties are influenced by flow oscillation at moderate to high frequencies. In addition, the data are also used to bring out the capability and limitations of quasi steady turbulence modelling in the prediction of unsteady shear flows. A further and more detailed analysis of the experimental data, results of some additional experiments and a discussion on the characterization of turbulent shear flows are provided in Part 2 (Ramaprian & Tu 1983). © 1983, Cambridge University Press. All rights reserved.
    Print ISSN: 0022-1120
    Electronic ISSN: 1469-7645
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
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