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  • 1
    Electronic Resource
    Electronic Resource
    Chromosome doubling in sterile Syringa vulgaris × S. pinnatifolia hybrids by in vitro culture of nodal explants (2000)
    Springer
    Plant cell, tissue and organ culture 63 (2000), S. 127-132 
    Details
    ISSN: 1573-5044
    Keywords: colchicine ; flow cytometry ; in vitro culture ; lilac
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The aim was to produce tetraploid forms of hybrids of Syringa vulgaris × S. pinnatifolia to restore fertility and enable further breeding to be undertaken. Excised nodal sections of three selections were pre-cultured for 5 days then treated with a range of colchicine concentrations for 1, 2 or 3 days, after which they were washed three times in sterile distilled water and cultured on shoot proliferation medium. Frequent movement to fresh medium was beneficial to survival. Three successive experiments established the range of concentrations of colchicine, 0.05 mM to 0.25 mM, at which tetraploids were likely to be produced. Thus a protocol for the production of tetraploids was established. Cytometric analysis showed that tetraploid forms of two selections were produced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006472101185
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  • 2
    Electronic Resource
    Electronic Resource
    Correlation of stylar ribonuclease isoenzymes with incompatibility alleles in apple (1999)
    Springer
    Euphytica 107 (1999), S. 29-43 
    Details
    ISSN: 1573-5060
    Keywords: apple ; incompatibility ; isoelectric focusing ; Malus pumila ; non-equilibrium pH gel electrofocusing ; ribonuclease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Fifty-six cultivars of apple were analysed for stylar ribonucleases; proteins were extracted from styles, separated by non-equilibrium pH gel electrofocusing and stained for activity. Excellent correlation was found between the ribonuclease bands revealed and the 11 known incompatibility, S, alleles, in 14 diploid cultivars genotyped in the classic work of Kobel by monitoring pollen tube growth after test crossing, and in 20 cultivars genotyped, at least partially, by more recent DNA methods. For 12 triploid cultivars studied by Kobel, the correlation was good but not perfect. Two apparent minor electrophoretic variants for S10 were noted and, to distinguish them from each other and also from the electrophoretically similar S3, isoelectric focusing was used. Ten cultivars were genotyped for the first time. In all, 14 ribonuclease bands that may correspond to the ‘new’ S alleles, S12 to S 25, were detected but these alleles should be regarded as provisional until confirmed by pollination tests, especially when the electrophoretic differences were only slight. Analysis of stylar ribonucleases is a convenient method of predicting S alleles in flowering material and thereby investigating incompatibility relationships. The polymorphism of the S locus makes it useful for checking the identity and parentage of cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003516902123
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  • 3
    Electronic Resource
    Electronic Resource
    Correlation of ribonuclease zymograms and incompatibility genotypes in almond (1997)
    Springer
    Euphytica 97 (1997), S. 167-176 
    Details
    ISSN: 1573-5060
    Keywords: Almond ; incompatibility ; non-equilibrium pH gradient electro-focusing ; Prunus dulcis ; ribonuclease ; self-compatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Proteins were extracted from styles of 29 self-incompatible cultivars of almond and separated using non-equilibrium pH gradient electro-focusing, and the gels were stained for ribonuclease activity. Mutually incompatible cultivars had similar banding patterns and, for the 24 cultivars already genotyped in France or California, the bands correlated well with the reported alleles. The band corresponding to S1 of the French labelling system was indistinguishable from that corresponding to Sb of the Californian labelling system, and a controlled cross confirmed that these alleles are identical. The band corresponding to the Californian Sa was distinct from the bands corresponding to French alleles and, to harmonise the allele labels, it was redesignated S5. The genotypes of five uncharacterised self-incompatible cultivars were inferred from zymograms as follows: ‘Desmayo Largueta’ and ‘Glorieta’, S1S5, ‘Masbovera’, S1S9, ‘Tarragones’, S2S9, and ‘Tokyo’, S6S7. The alleles designated S6 and S9 have not previously been reported. Nine self-compatible cultivars or selections were analysed, and each showed a band corresponding to an incompatibility allele as well as a common band; however, the correspondence of this common band to Sf, the allele for self-compatibility, is unproven.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003030913308
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  • 4
    Electronic Resource
    Electronic Resource
    Inheritance of stylar ribonucleases in cherry progenies, and reassignment of incompatibility alleles to two incompatibility groups (1997)
    Springer
    Euphytica 95 (1997), S. 221-228 
    Details
    ISSN: 1573-5060
    Keywords: cherry ; genetics ; incompatibility ; isoelectric focusing ; Prunus avium ; ribonuclease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Stylar proteins were extracted from parents and seedlings of six progenies of cherry (Prunus avium), separated using isoelectric focusing, and the gels stained for ribonuclease activity. The zymogram of each plant showed two main ribonuclease bands in the region pI 8.3 to 9.6. Progenies from crosses of parents with one band in common segregated into just two classes, whereas progenies from crosses of parents with no common bands segregated into four classes, the two types of segregation corresponding to those expected from semi-compatible and fully-compatible crosses respectively. This behaviour was consistent either with the ribonuclease locus being tightly linked with the self-incompatibility, S, locus, or else with the S locus coding for the ribonuclease variants. Evidence favouring the latter hypothesis is discussed. An apparently anomalous segregation led us to assign to ‘Bradbourne Black’ a genotype different from that previously reported, and analysis of some other cultivars in the same incompatibility group, Group VII, led us to conclude the genotype of this group is S3S5, and not S4S5 as previously reported. Correspondingly, we suggest the genotype of Group V is S4S5, and not S3S5. Five new S alleles, S7, S8, S9, S10 and S11 were proposed in parental cultivars and selections that had not previously been assigned a genotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002945529157
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  • 5
    Electronic Resource
    Electronic Resource
    Inheritance and linkage relationships of isoenzymes in two interspecific cherry progenies (1998)
    Springer
    Euphytica 103 (1998), S. 273-286 
    Details
    ISSN: 1573-5060
    Keywords: cherry ; genetics ; isoenzymes ; linkage ; mapping ; Prunus avium ; P. incisa ; P. nipponica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two interspecific cherry progenies, Prunus avium ‘Napoleon’ × P. incisa E621 and ‘Napoleon’ × P. nipponica F1292, were analysed with polyacrylamide gel electrophoresis for 19 enzyme systems: alanine aminopeptidase (AAP), aldolase (ALD), alkaline phosphatase (AKP), arginine aminopeptidase (ARA), catechol oxidase (CO), diaphorase (DIA), endopeptidase (ENP), esterase (EST), formate dehydrogenase (FDH), fructose-bisphosphatase (FBP), β-galactosidase (GAL), glucose-6-phosphate dehydrogenase (G6PDH), β-glucosidase (GLU), glutamate dehydrogenase (GDH), glyceraldehyde-3-phosphate dehydrogenase (G3PDH), hexokinase (HEX), peroxidase (PRX), phosphorylase (PHO) and triose-phosphate isomerase (TPI). Activity for GAL was identical with GLU, and CO with PRX. In addition, activity for AKP was identical with some regions detected previously as acid phosphatase; and most of the PRX activity was identical with regions detected previously as superoxide dismutase. In all, 16 new segregating loci were identified, Ara-1, Dia-2, Est-1, Est-2, -6, -7 and -9, Glu-1 to -4, Hex-1, Pho-1 and -2, and Prx-8 and -9, together with 10 polymorphic putative loci. Analysis of cosegregations of the segregating loci with each other, and with loci established previously, resulted in 19 more loci being added to the cherry linkage map. Fifteen of these were new, and four had been described previously, but were hitherto unlinked. The additional linkages are: Ara-1– Dia-2– Mdh-2–Est-2; Glu-3/Prx-8–Glu-1/-2/-4–Prx-1/-9; Pgd-2–Pho-1/-2; Idh-2–Est-7–Est-6; (Amy-2)–Est-1–(Adh-4/-6); and (Acp-1/-2/-3)–Hex-1–(Gpi-2). (The bracketed loci had been mapped already). Several cherry linkages resemble linkages reported in apple. Analysis of 14 cultivars of P. avium for the same enzyme systems revealed polymorphism for just four of the 15 loci, and for an additional putative locus that was monomorphic in the interspecific material. This lack of isoenzyme polymorphism among cherry cultivars reduces the utility of these markers for linkage analysis and mapping in progenies of P. avium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018664600714
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  • 6
    Electronic Resource
    Electronic Resource
    Inheritance of isoenzymes and their linkage relationships in two interspecific cherry progenies (1997)
    Springer
    Euphytica 93 (1997), S. 129-143 
    Details
    ISSN: 1573-5060
    Keywords: cherry ; genetics ; isoenzymes ; linkage ; Prunus avium ; Prunus incisa ; Prunus nipponica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two interspecific cherry progenies, Prunus avium 'Napoleon' × P. incisa E621 and × P. nipponica F1292, were analysed by polyacrylamide gel electrophoresis for 14 enzyme systems: aconitase (ACO), acid phosphatase (ACP), alcohol dehydrogenase (ADH), amylase (AMY), glutamate oxaloacetate transaminase (GOT), glucose–6-phosphate isomerase (GPI), isocitrate dehydrogenase (IDH), leucine aminopeptidase (LAP), malate dehydrogenase (MDH), malic enzyme (ME), phosphogluconate dehydrogenase (PGD), phosphoglucomutase (PGM), shikimate dehydrogenase (SKD) and superoxide dismutase (SOD). Thirty-one loci were deduced from segregating banding patterns, Aco–2, Acp–1 to –5, Acp–8, –9, Adh–1 to –6, Amy–2, –3, Got–1 to –3, Gpi–2, Idh–1 to –4, Lap–1, Me–1, –2, Mdh–2, Pgd–1, –2 and Sod–2. Only ten of these had previously been established. Seven putative loci were polymorphic but did not segregate in the progenies. Analysis of cosegregations and calculation of recombination fractions revealed that 15 loci could be grouped into four linkage groups: Acp–1/–2/–3–-Acp–5; Gpi–2–- Got–2–-Got–1–-Lap–1; Adh–4/–6– -Amy–2; and Adh–1–-Adh–5–-Adh–2–- Me–2. These consolidate two previously reported linkage groups and establish three new groups. The previously reported linkage of Lap–1 with Me–1 was not confirmed. Fourteen cultivars of P. avium were analysed for the same 14 enzyme systems and showed polymorphism for just 17 of the established loci and for none of the putative loci, indicating far less scope for linkage analysis in intraspecific progenies from crosses among these cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002959425639
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