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  • 1
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    ZZW autotriploidy in a Blue-and-Yellow Macaw (1991)
    Springer
    Details
    Publication Date: 1991-01-01
    Print ISSN: 0016-6707
    Electronic ISSN: 1573-6857
    Topics: Biology
    Published by Springer
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    PAPER CURRENT
  • 2
    Electronic Resource
    Electronic Resource
    Transport and cryopreservation of sperm of the common snook, Centropomus undecimalis (Bloch) (2004)
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 35 (2004), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Sperm were collected in Florida from wild common snook, Centropomus undecimalis (Bloch), and were shipped to Louisiana State University for analysis and cryopreservation. Threshold activation of sperm (10% motility) occurred at 370 mOsmol kg−1, and complete activation occurred at 680 mOsmol kg−1. These values were significantly different. Sperm samples stored at 1°C in Hanks' balanced salt solution (HBSS) or in 0.6% NaCl solution at 200 mOsmol kg−1 retained motility for as long as 22 days. Mean motility remained above 50% for 9 days for sperm stored in HBSS and for 7 days for sperm stored in NaCl solution. Sperm exposed to 5% dimethyl acetamide (62±10%; mean±SD), 10% dimethyl sulphoxide (DMSO) (39±16%), 5% glycerol (26±5%) or 10% glycerol (6±2%) for 30 min had significantly lower motility than did unexposed sperm (89±9%). When used as a cryoprotectant, samples frozen with 5% or 10% DMSO or 5% methanol had significantly higher post-thaw motility than did samples frozen with other cryoprotectants. Sperm cryopreserved with 10% DMSO (38±12%) had significantly higher post-thaw motility than did sperm cryopreserved with 15% DMSO (19±10%) or 20% DMSO (4±4%). There were no significant differences in hatch rates of eggs fertilized with fresh sperm (54±29%) or cryopreserved sperm (41±35%). Survival to first feeding was not different between fish produced with fresh sperm (37±30%; range, 0–86%) or with thawed sperm (24±29%; 0–77%). Transport of sperm to a cryopreservation laboratory and back to a hatchery for thawing and use enabled collaboration between groups with specific expertise and provides a model for the application of cryopreservation by transport of fresh and frozen samples.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2109.2004.01013.x
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  • 3
    Electronic Resource
    Electronic Resource
    Refrigerated storage and cryopreservation of sperm of red drum, Sciaenops ocellatus L. (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 29 (1998), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: To aid in artificial spawning of sciaenid fishes, the present authors developed techniques to collect, handle and cryopreserve sperm from red drum, Sciaenops ocellatus L. Sperm were collected by removing and slicing the testis, and adding Hanks' balanced salt solution (HBSS) or NaCl solution (each at 200-400 mOsm kg−1) as an extender. Sperm were activated with 800 mOsm kg−1 artificial sea water (ASW) to characterize motility. Sperm reached maximum motility (highest percentage motility observed for that sample) within 8 ± 1 s (mean ± SD) and remained at maximum motility for 33 ± 4 s. Sperm were exposed to graded osmotic pressures of ASW (8-800 mOsm kg−1) to determine the range of osmolalities that elicited motility. Threshold activation (defined as ∼10% motility) occurred at 351 ± 4 mOsm kg−1 and complete activation occurred at 539 ± 2 mOsm kg−1. Sperm stored at 200 mOsm kg−1 retained motility for up to 13 days. Dimethyl sulphoxide (DMSO) was used as a cryoprotectant at concentrations ranging from 7.5% to 15% (v:v) in HBSS (200 mOsm kg−1). There were no significant differences among post-thaw motilities of sperm cryopreserved at any concentration of DMSO. Sperm thawed on the benchtop at 21°C had lower post-thaw motility than did sperm thawed at 10, 20, 30, 40, 50 or 60°C in a water bath.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.1998.00198.x
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  • 4
    Electronic Resource
    Electronic Resource
    Four members of the Sox gene family in channel catfish (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 58 (2001), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The homologous sequences of human or mouse SOX1, SOX4 and SOX11, and one novel Sox gene (named Ccf-SoxN) were identified in the genome of channel catfish Ictalurus punctatus. Identification of these genes is a potential step in understanding development regulations including sex determination in channel catfish.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.2001.tb00541.x
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  • 5
    Electronic Resource
    Electronic Resource
    Chromosomal inheritance patterns of intergeneric hybrids of ictalurid catfishes: odd diploid numbers with equal parental contributions (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 51 (1997), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Hybrid chromosomal compositions of channel catfish Ictalurus punctatus× black bullhead Ameiurus melas and channel catfish × flathead catfish Pylodictis olivaris were analysed by a computer-based method. The karyotype of each hybrid was highly asymmetric, and the diploid numbers and arm numbers were intermediate to the parental types. The hybrid offspring of channel catfish × black bullhead possessed a diploid number of 59 chromosomes, with an arm number estimate of 87. The hybrid offspring of the channel catfish × flathead catfish cross possessed a diploid number of 57 chromosomes, also with an arm number estimate of 87. Nucleolus organizer regions (NORs) were located on a single pair of chromosomes with symmetric staining intensity in channel catfish and in black bullhead, and on a single pair of chromosomes with asymmetric staining intensity in flathead catfish. The channel catfish × black bullhead hybrid had two unpaired chromosomes that stained positively for NORs. The channel catfish × flathead catfish had three unpaired chromosomes that stained positively for NORs. Specific marker chromosomes were identified in each hybrid. There was no evidence of androgenesis, gynogenesis, polyploidy or aneuploidy in the hybrids. Results of this study, plus information reported previously, indicate that chromosomes of ictalurid catfishes are inherited stably in a haploid pattern with an equal contribution to the genomes of F1 hybrids, even in intergeneric crosses involving divergent numbers of parental chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1997.tb01128.x
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  • 6
    Electronic Resource
    Electronic Resource
    Chromosomal location of the 28S ribosomal RNA gene of channel catfish by in situ polymerase chain reaction (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 56 (2000), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: This study describes the use of the polymerase chain reaction for physical mapping of fish genes. A 287–base pair (bp) fragment of the 28S ribosomal RNA gene (28S rDNA) of channel catfish Ictalurus punctatus was isolated and sequenced with human-derived primers. The nucleotide (nt) sequence of this fragment was 20 bp shorter than that of the corresponding region of the human 28S rDNA. The gene was mapped to chromosomes of channel catfish by fluorescence in situ hybridization (FISH) and in situ polymerase chain reaction (ISPCR). A major locus and a minor locus of 28S rDNA were found on chromosomes of channel catfish. The major locus was associated with the active nucleolus organizer region (NOR) sites. The minor locus was highly resolved and not detectable by silver staining, suggesting that this locus was not involved in synthesis of ribosomal RNA and possessed fewer copies of 28S rDNA. Both loci contained GC-rich DNA elements that could be components of 28S rDNA repeated units. In this study, a potential method of comparative mapping of the channel catfish genome has been presented by using human-derived oligonucleotide sequences. These data demonstrate that ISPCR is highly specific and will be useful in physical mapping of fish genomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.2000.tb02113.x
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  • 7
    Electronic Resource
    Electronic Resource
    Cryopreservation of leucocytes of channel catfish for subsequent cytogenetic analysis (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 47 (1995), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Channel catfish leucocytes cryopreserved with glycerol or dimethyl sulphoxide (DMSO) had significantly higher (P〈0.05) viability and recovery rates than did cells cryopreserved with methanol. After 7 days of frozen storage, a 24 to 27% reduction of viability was observed for cells cryopreserved with glycerol; a 25 to 43% reduction for cells frozen with DMSO, and a 67 to 100% reduction for cells frozen with methanol. The concentration of cryoprotectants affected the viability of cryopreserved cells significantly (p〈0.05). The viability reduction was 36% for cells frozen with 5% of cryoprotectants, 30% for cells frozen with 10% of cryoprotectants, and 49% for cells frozen with 15% of cryoprotectants. The viability of cells frozen at the slower rate (-2.7°C min−1) was significantly higher (p〈0.05) than that of cells frozen at the faster rate (-45°C min−1). Best results were obtained for cells cryopreserved with 10% of glycerol or DMSO and frozen at the slower rate. The chromosomes prepared from cells cryopreserved using this procedure were identical to those prepared from fresh cells, and to those reported in the literature for channel Catfish.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1995.tb06025.x
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  • 8
    Electronic Resource
    Electronic Resource
    Effects of Environmental Sodium Chloride on Percent Hatch, Yolk Utilization, and Survival of Channel Catfish Fry (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the World Aquaculture Society 28 (1997), S. 0 
    Details
    ISSN: 1749-7345
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract.— Only limited research has addressed the effect of salinity on hatching of channel catfish Ictalurus punctatus eggs, and no studies have evaluated the effect of salinity on fry development and survival. This study was undertaken to determine the effect of environmental sodium chloride (0, 1, 2, and 4 g/L. NaCl) on percent hatch, yolk utilization, and survival of channel catfish fry. Experiments were conducted in recirculating systems using seven egg masses (1–2 d old). Each egg mass was divided into smaller portions which remained undissociated or were dissociated with sodium sulfite (NaSO3). Eggs were incubated until hatching. Wet and dry weights were obtained for sacfry at 1 and 5 d post-hatch to determine wet weight gain and dry weight loss, and fry were sampled 7 d after initiation of exogenous feeding to determine survival. Percent hatch, yolk utilization, and survival of fry hatched from undissociated eggs were greatest at 1 g/L NaCl. In addition, treatment of eggs with NaSO3 significantly reduced percent hatch at all NaCl levels. Although our results indicate that addition of NaCI to hatchery water supplies can increase production of channel catfish fry, additional research is needed before this practice can be recommended on a commercial basis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-7345.1997.tb00645.x
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  • 9
    Electronic Resource
    Electronic Resource
    ZZW autotriploidy in a Blue-and-Yellow Macaw (1991)
    Springer
    Genetica 84 (1991), S. 209-212 
    Details
    ISSN: 1573-6857
    Keywords: Triploidy ; genome size ; Ara ararauna ; flow cytometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We describe genome size (nuclear DNA content), and cellular and nuclear dimensions of erythrocytes in a triploid Blue-and-Yellow Macaw (Ara ararauna) and its diploid parents. The genome size of the triploid (4.23 pg) was 1.5 times greater than the genome size of the mother (2.80 pg) and the father (2.89 pg). The sex chromosome composition was ZZW, and was predicted correctly based on the genome size of the parents. Erythrocytes of the triploid were significantly larger than the erythrocytes of the parents. Because polyploidy has been reported only in one other family of birds (Phasianidae), the parrots and their relatives might prove to be useful in the study of avian triploidy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00127249
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  • 10
    Electronic Resource
    Electronic Resource
    Histochemical and biochemical studies of the hepatopancreas peroxidase of the freshwater crayfish, Cambarus robustus (1985)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 184 (1985), S. 171-182 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Crayfish are among the few invertebrate species reported to possess endogenous peroxidase activity. The enzyme is found within the hepatopancreas, the principal digestive and absorptive organ of the crustacean body. Cambarus robustus, a species found in abundance in the streams of western New York, was used in this study. Homogenates of 18 hepatopancreases were assayed for peroxidase activity using guaiacol as the substrate. Although present in all organs, peroxidase activity displayed a greater than 50-fold difference between the two extremes (0.05-;2.72 units/mg protein). Histochemical examination using diaminobenzidine revealed peroxidase activity within a line of cells extending along the distal two-thirds of the lengths of all hepatopancreatic tubules. The cells function to synthesize the enzyme, sequester it within vacuoles of increasing size, and eventually secrete it into the tubule lumen. Since the tubule is constantly renewed by distal mitotic activity and concomitant proximal exfoliation, this histochemical technique permits not only the examination of the ontogeny of this peroxidase-positive cell line, but also offers additional insight into the mechanism of hepatopancreatic tubule renewal.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051840207
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