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  • 1
    Electronic Resource
    Electronic Resource
    Hygromycin resistance is an effective selectable marker for biolistic transformation of black spruce (Picea mariana) (2000)
    Springer
    Plant cell reports 19 (2000), S. 358-362 
    Details
    ISSN: 1432-203X
    Keywords: Key words Picea mariana ; Conifer transformation ; Biolistics ; Hygromycin selection ; Transgenic trees
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Using particle bombardment of mature somatic embryos followed by the induction of secondary embryogenesis in the presence of hygromycin, we produced over 90 lines of transgenic embryonal masses expressing β-glucuronidase from two genotypes of black spruce. Transformation efficiencies of up to 7% (1 transgenic line per 14 embryos bombarded) were achieved by extending the period of selection from 8 to 12 weeks. Proliferation of transformed embryonal masses in the presence of hygromycin had no effect on either embryogenicity or embryo maturation. Southern blot hybridization and PCR amplification confirmed the presence of the hygromycin phosphotransferase gene in genomic DNA. The expression of the β-glucuronidase gene in the needles of regenerated seedlings support the potential for long-term transgene expression in spruce.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050740
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  • 2
    Electronic Resource
    Electronic Resource
    In vitro response and pedigree analysis for somatic embryogenesis of long-day photoperiod adapted soybean (1994)
    Springer
    Plant cell, tissue and organ culture 36 (1994), S. 269-273 
    Details
    ISSN: 1573-5044
    Keywords: Glycine max ; genotype ; high-latitude adapted ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Northern-adapted soybean cultivars were screened for their ability to form somatic embryos in vitro in response to exposure to 180 μM 2,4-dichlorophenoxyacetic acid in a modified Murashige & Skoog (1962) medium containing B5 (Gamborg et al. 1968) organics and 43.5 mM sucrose. The 20 cultivars formed between 1 and 7 embryos per cotyledon with between 18% and 98% of the cotyledons responding. The response was genotype dependant. Three ancestral lines, ‘840-7-3’, ‘A.K. Harrow’ and ‘Mandarin’ were prominent in the genetic background of long-photoperiod adapted lines and showed a high degree of somatic embryogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037731
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    Paper (German National Licenses)
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