Publication Date:
2018-11-29
Description:
Introduction: Interleukin 6 (IL-6) is proinflammatory cytokine which is produced by cell when Nod-like receptors (NLRs) are activated. Increased production of IL-6 was shown to promote tumor growth and metastasis.Therefore, it could be suggested that activation of NLRs could support malignancy. PC-3, prostate tumor derived cells, was shown to produce IL-6; however, little is known about the role of NLRs in regulation of cytokine secretion. Method: PC-3 cells were seeded in 48 well plate cell culture plates and culturd in presence of VX765, Nigericin or Camptothesin. After 24 hours incubation, cell death was determined using Annexin V test. Additionally, IL-6 level was assessed in culture medium using Bio-Plex Pro™ Human Chemokine Panel (Biorad). Data was analyzed using One Way Anova, Post Hoc Tukey test, SPSS 20. Results: Viability of PC3 cells was reduced when treated with Nigericin (37.74±0.33%) and Champtothesin (10.10±0.23%) as compared to untreated cells (P
Print ISSN:
0006-4971
Electronic ISSN:
1528-0020
Topics:
Biology
,
Medicine
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