ISSN:
1618-2650
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A method for preparative isolation of serum lipoproteins by a combination of differential and density gradient ultracentrifugation is presented. Total plasma lipoproteins are first isolated in a concentrated form by ultracentrifugation in a fixed angle rotor at a plasma background density of 1.21 kg/l. Subsequently, the various lipoprotein classes are separated by density gradient ultracentrifugation in a swinging bucket rotor. The procedure requires only two ultracentrifugation steps and combines advantages of both ultracentrifugation techniques.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00470729
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