ISSN:
1573-4927
Keywords:
storage protein
;
evolution
;
napin
;
matteuccin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract The ostrich fern,Matteuccia struthiopteris L., contains two globulin spore storage proteins of 2.2 S and 11.3 S, with physical characteristics similar to those of seed storage proteins ofBrassica napus (rapeseed) andRaphanus sativus (radish). By the use of a cloned cDNA that encodes the 1.7 SB. napus storage protein (napin), gene sequences that hybridized with napin were detected in fern nuclear DNA, and a 900-nucleotide homologous mRNA was detected in developing spores.In vitro translation of this fern mRNA produced a 22-kD polypeptide comparable in size to the 21-kD precursor polypeptide identified inBrassica. No hybridizations were observed between theBrassica 12 S clone and either fern DNA or developing spore mRNA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00553782
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