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  • 1
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    Graphite: a mimic for DNA and other biomolecules in scanning tunneling microscope studies (1991)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1991-02-18
    Description: Highly ordered pyrolytic graphite (HOPG) is the substrate often used in scanning tunneling microscope (STM) studies of biomolecules such as DNA. All of the images presented in this article are of freshly cleaved HOPG surfaces upon which no deposition has occurred. These images illustrate features previously thought to be due to biological molecules, such as periodicity and meandering of "molecules" over steps. These features can no longer be used to distinguish real molecules from features of the native substrate. The feasibility of the continued use of HOPG as a substrate for biological STM studies is discussed.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Clemmer, C R -- Beebe, T P Jr -- S07RR07092/RR/NCRR NIH HHS/ -- New York, N.Y. -- Science. 1991 Feb 8;251(4994):640-2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry, University of Utah, Salt Lake City 84112.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1992517" target="_blank"〉PubMed〈/a〉
    Keywords: Adsorption ; DNA/*ultrastructure ; Graphite/*chemistry ; Microscopy, Electron, Scanning/*methods ; Models, Structural ; Periodicity
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    "Molecule corrals" for studies of monolayer organic films (1994)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1994-07-08
    Description: Scanning tunneling microscopy (STM) studies have demonstrated that monolayer-deep, flat-bottomed, circular etch pits can be grown on highly ordered pyrolytic graphite by high-temperature etching in the presence of oxygen. In this work, these graphite etch pits are used as "molecule corrals" to isolate ensembles of molecules for study by STM. The nucleation of self-assembled molecular films in the corrals took place by nucleation events separate from those leading to self-assembly on the surrounding terrace and allowed the measurement of the nucleation rate constant in the corrals. The dependence of the nucleation rate for self-assembly on pit size shows that nucleation occurs at open terrace sites and that step edges (that is, the corral's perimeter) and confinement inhibit film growth.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Patrick, D L -- Cee, V J -- Beebe, T P Jr -- New York, N.Y. -- Science. 1994 Jul 8;265(5169):231-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17750666" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 3
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    Direct observation of native DNA structures with the scanning tunneling microscope (1989)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1989-01-20
    Description: Uncoated double-stranded DNA dissolved in a salt solution was deposited on graphite and imaged in air with the scanning tunneling microscope (STM). The resolution was such that the major and minor grooves could be distinguished. The pitch of the helix varied between 27 and 63 angstroms in the images obtained. Thus the STM can be useful for structural studies of a variety of uncoated and isolated biomolecules.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Beebe, T P Jr -- Wilson, T E -- Ogletree, D F -- Katz, J E -- Balhorn, R -- Salmeron, M B -- Siekhaus, W J -- New York, N.Y. -- Science. 1989 Jan 20;243(4889):370-2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Lawrence Livermore National Laboratory, Department of Chemistry, Livermore, CA 94550.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2911747" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cattle ; DNA/*ultrastructure ; Microscopy, Electron, Scanning/instrumentation/*methods ; *Nucleic Acid Conformation
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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