Publikationsdatum:
1995-12-15
Beschreibung:
The relative locations of several chromosomes within wheel-shaped prometaphase chromosome rosettes of human fibroblasts and HeLa cells were determined with fluorescence hybridization. Homologs were consistently positioned on opposite sides of the rosette, which suggests that chromosomes are separated into two haploid sets, each derived from one parent. The relative locations of chromosomes on the rosette were mapped by dual hybridizations. The data suggest that the chromosome orders within the two haploid sets are antiparallel. This chromosome arrangement in human cells appears to be both independent of cell type- and species-specific and may influence chromosome topology throughout the cell cycle.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nagele, R -- Freeman, T -- McMorrow, L -- Lee, H Y -- New York, N.Y. -- Science. 1995 Dec 15;270(5243):1831-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biology, University of Medicine and Dentistry of New Jersey, School of Osteopathic Medicine, Stratford 08084, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8525379" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Cells, Cultured
;
Chromosomes/*physiology/ultrastructure
;
DNA Probes
;
Fibroblasts/ultrastructure
;
HeLa Cells
;
Humans
;
In Situ Hybridization, Fluorescence
;
Metaphase/genetics/*physiology
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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