ISSN:
1662-8985
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
This paper describes about microbial diversity in an iron oxidation tank of an AMDtreatment plant established at an abandoned sulphur mine in Japan. Since the mining operation wasstopped, this mine has produced strong acidic mine drainage, pH 1.8, including a high concentrationof ferrous iron, 301mg/L, and the flow rate was about 4m3/min. In 2006, a pilot scale microbial ironoxidizing system was installed to remove total iron more easily from the AMD by oxidizing ferrousiron to ferric iron. From the start of this pilot operation, microbial diversity in the iron oxidationtank was investigated using a PCR-DGGE method for about two months. In the PCR, V3 region of16S rRNA gene for Bacteria was amplified. The profiles of DGGE showed that there were threedominant species in the iron oxidation tank through the experimental period. Number of bands onDGGE profiles decreased with dates of sampling so that the microbial population became lessdiverse because of the iron oxidizing operation. There was a wide variety of bacterial species ofeven though conditions were strongly acidic
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/39/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.20-21.493.pdf
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