ALBERT

Description: The Specimen Chamber Service Unit, a component of the Space Station Centrifuge Facility, must provide a clean enclosure on a continuing basis for the facility's plant, rodent and primate specimens. The specimen chambers can become soiled and can require periodic servicing to maintain a clean environment for the specimens. Two methods of servicing the specimen chambers are discussed: washing the chambers with an on-board washer, or disposing of the soiled chambers and replacing them with clean ones. Many of these issues are addressed by developing several servicing options, using either cleaning or replacement as the method of providing clean specimen chambers, and then evaluating each option according to a set of established quantitative and qualitative criteria. Disposing and replacing the Specimen Chambers is preferable to washing them.

Description: The Life Sciences Glovebox will provide the bioisolated environment to support on-orbit operations involving non-human live specimens and samples for human life sceinces experiments. It will be part of the Centrifuge Facility, in which animal and plant specimens are housed in bioisolated Habitat modules and transported to the Glovebox as part of the experiment protocols supported by the crew. At the Glovebox, up to two crew members and two habitat modules must be accommodated to provide flexibility and support optimal operations. This paper will present several innovative design concepts that attempt to satisfy the basic Glovebox requirements. These concepts were evaluated for ergonomics and ease of operations using computer modeling and full-scale mockups. The more promising ideas were presented to scientists and astronauts for their evaluation. Their comments, and the results from other evaluations are presented. Based on the evaluations, the authors recommend designs and features that will help optimize crew performance and facilitate science accommodations, and specify problem areas that require further study.

Description: It has been shown that prohibitive resupply costs for extended-duration manned space flight missions will demand that a high degree of recycling and in situ food production be implemented. A prime candidate for in situ food production is the growth of higher level plants. Research in the area of plant physiology is currently underway at many institutions. This research is aimed at the characterization and optimization of gas exchange, transpiration and food production of higher plants in order to support human life in space. However, there are a number of unresolved issues involved in making plant chambers an integral part of a closed life support system. For example, issues pertaining to the integration of tightly coupled, non-linear systems with small buffer volumes will need to be better understood in order to ensure successful long term operation of a Controlled Ecological Life Support System (CELSS). The Advanced Life Support Division at NASA Ames Research Center has embarked on a program to explore some of these issues and demonstrate the feasibility of the CELSS concept. The primary goal of the Laboratory Scale CELSS Project is to develop a fully-functioning integrated CELSS on a laboratory scale in order to provide insight, knowledge and experience applicable to the design of human-rated CELSS facilities. Phase I of this program involves the integration of a plant chamber with a solid waste processor. This paper will describe the requirements, design and some experimental results from Phase I of the Laboratory Scale CELSS Program.

Description: The document is a 2 page fact sheet that describes the Bioculture system, how it may be used by researchers for life science research, how and when it will be installed and validated aboard the international space station.

Description: Future human exploration missions will require life support systems that are highly regenerative, requiring minimum resupply, enabling the crews to be largely self-sufficient. Solid wastes generated in space will be processed to recover usable material. Researchers at NASA Ames Research Center are studying a commercially-produced microwave incinerator as a solid waste processor. This paper will describe the results of testing to-date.

Description: The handling and disposal of solid waste matter that has biological or biohazardous components is a difficult issue for hospitals, research laboratories, and industry. NASA faces the same challenge as it is developing regenerative systems that will process waste materials into materials that can be used to sustain humans living in space for extended durations. Plants provide critical functions in such a regenerative life support scheme in that they photosynthesize carbon dioxide and water into glucose and oxygen. The edible portions of the plant provide a food source for the crew. Inedible portions can be processed into materials that are more recyclable. The Advanced Life Support Division at NASA Ames Research Center has been evaluating a microwave incinerator that will oxidize inedible plant matter into carbon dioxide and water. The commercially available microwave incinerator is produced by Matsushita Electronic Instruments Corporation of Japan. Microwave incineration is a technology that is simple, safe, and compact enough for home use. It also has potential applications for institutions that produce biological or biohazardous waste. The incinerator produces a sterile ash that has only 13% of the mass of the original waste. The authors have run several sets of tests with the incinerator to establish its viability in processing biological material. One goal of the tests is to show that the incinerator does not generate toxic compounds as a byproduct of the combustion process. This paper will describe the results of the tests, including analyses of the resulting ash and exhaust gases. The significance of the results and their implications on commercial applications of the technology will also be discussed.

Description: NASA invests in professional coaching as a way to accelerate the development of its staff. The speaker shares one foundational human development model in coaching - the Six Streams - and applies it to the challenges that new scientists face. The speaker also describes how a new scientist can develop greater capabilities in the Six Streams so that they can become a more effective scientist and feel more satisfaction with their work.

Description: Forest development patterns following disturbance are known to influence the physical and chemical attributes of soils at different points in time. Changes in soil resources are thought to have a corresponding effect on ectomycorrhizal (ECM) community structure. We used molecular methods to compare below-ground ECM species richness, composition, and abundance between adjacent stands of homogenous lodgepole pine and old growth mixed conifer in Yellowstone National Park (YNP). In each stand-type we collected soil cores to both identify mycorrhizae and assess soil chemistry. Although no statistical difference was observed in the mean number of ECM root tips per core between stand types, the total number of species identified (85 versus 35) and the mean number of species per core (8.8 +/- 0.6 versus 2.5 +/- 0.3) were significantly higher in lodgepole pine. Differences between the actual and estimated species richness levels indicated that these forest types support a high number of ECM species and that undersampling was severe. Species compositions were widely disparate between stands where only four species were shared out of a total of 116. Soil analysis also revealed that mixed conifer was significantly lower in pH, but higher in organic matter, potassium, phosphorus, and ammonium when compared to lodgepole pine stands. Species richness per core was correlated with these chemical data, however, analysis of covariance indicated that stand type was the only statistically significant factor in the observed difference in species richness. Our data suggest that ECM fungal richness increases as homogenous lodgepole pine stands grow and mature, but declines after Engelmann spruce and subalpine fir colonize. Despite difficulties linking species composition with soil chemistry, there are a variety of physical and chemical factors that could be influencing ECM community structure. Future field experiments are necessary to test some of the mechanisms potentially operating within this system.

Description: Crew operations associated with nonhuman life sciences research on Space Station Freedom will be conducted in the Life Sciences Glovebox, whose enclosed work volume must accommodate numerous life science procedures. Two candidate Glovebox work volume concepts have been developed: one in which two operators work side-by-side, and another that conforms to the reach envelope of a single operator. Six test volunteers tested the concepts according to preestablished operational criteria. The wrap-around, single-operator concept has been judged the superior system.