ISSN:
0884-3996
Keywords:
Streptavidin
;
biotin
;
chemiluminescence
;
universal marker
;
immunoassay
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
The tetrameric structure of streptavidin and its exceptionally strong affinity to biotin (Ka = 1015M-1) can be exploited to achieve an amplification of the signal in immunoassays. In the approach described here streptavidin (STAV) labelled with aminobutylethyl-isoluminol (ABEI) served as a universal marker in immunoassays for both haptens and big antigens. The advantageous features of streptavidin can be applied to any immunoassay using biotinylated antibodies as the primary probe.In two-site immunometric assays for larger antigens the liquid phase ‘tracer’ antibody is biotinylated. In hapten assays the solid phase antigen technique (Wood et al., 1982) is employed, in which sample-antigen and solid phase-antigen compete for a biotinylated antibody. In this paper we demonstrate the use of STAV-ABEI as a universal chemiluminescent label in steroid assays and in an immunometric assay using human growth hormone (hGH) as an example.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bio.1170040117
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