Publication Date:
2016-09-17
Description:
The FLP/FRT system permits rapid phenotypic screening of homozygous lethal mutations in the context of a viable mosaic fly. Combining this system with ovo D dominant female-sterile transgenes enables efficient production of embryos derived from mutant germline clones lacking maternal contribution from a gene of interest. Two distinct sets of FRT chromosomes, carrying either the mini- white ( w +mW.hs ), or rosy ( ry + ) and neomycin ( neo R ) transgenes are in common use. Parallel ovo D lines were developed using the w +mW.hs FRT insertions on the X and chromosomes 2R and 3L, as well as the ry + , neo R FRT insertions on 2L and 3R. Consequently, mutations isolated on the X, 2R and 3L chromosomes in a ry + , neo R FRT background, are not amenable to germline clonal analysis without labor-intensive recombination onto chromosome arms containing a w +mW.hs FRT . Here we report the creation of a new ovo D line for the ry + , neo R FRT insertion at position FRT42D on chromosome 2R, through induced recombination in males. To establish the developmental relevance of this reagent we characterized the maternal-effect phenotypes of novel brother of tout-velu alleles generated on an FRT42D chromosome in a somatic mosaic screen. We find that an apparent null mutation that causes severe defects in somatic tissues has a much milder effect on embryonic patterning, emphasizing the necessity of analyzing mutant phenotypes at multiple developmental stages. This article is protected by copyright. All rights reserved.
Topics:
Biology
,
Medicine
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