ISSN:
1432-1211
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract We have used the human teratocarcinoma-derived embryonal carcinoma cell line Tera-2 cl. 13 to explore the putative expression of novelHLA class I(-like) genes. Serological analyses revealed that Tera-2 cells do not express polymorphic HLA class I (−A, −B, −C) specificities, but do express HLA class I-like antigens. These phenotypic properties parallel those of certain mouse embryonal carcinoma cells. To study the expression ofHLA class I(-like) genes in the Tera-2 cells two different approaches were used. Screening ofα Tera-2 cDNA library with a full-length HLA class I cDNA probe under conditions that would allow for the identification of relatively distinct HLA class I-like sequences yielded 27 positive clones, all of which were of the regular HLA-A, -B, -C type. Reverse northern hybridizations of the restriction enzyme-digestedTla bregion comprising cosmids with Tera-2 cDNA as the probe resulted in the identification of several putative human genes whose equivalents map within the mouseTla region. However, none of these genes appeared to be structurally related toHLA class I. A putativeH3.3 histone gene was identified in the proximalTla region of the C57BL/10 mouse. It is concluded that no structural homologues of mouseQa/Tla genes are expressed in the human developmental cell line Tera-2.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02114972
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