ISSN:
1432-1017
Keywords:
Fluorescence
;
energy transfer
;
ribosome
;
tRNA
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Physics
Notes:
Abstract Using the technique of singlet-singlet (Förster-type) resonance energy transfer, we have determined five distances in the programmed ribosome, either with the P site or with both the A and the P sites occupied. Two of the distances are new and two agree with earlier measurements; the fifth showed disagreement in detail with earlier results of others, but a consistent general trend. The distances substantiate a current model for the location of ribosomally bound tRNA, except in regard of the position of the 3′ end of P-site tRNA, which seems according to our results to lie too far away from the 3′ terminus of the 16 S RNA to be accomodated in the model. We present new evidence for the hypothesis that anomalously charged tRNA does not bind to the cognately programmed A site in the same way as does tRNA charged with an amino acid. Occupation of the A site restricts mobility of the 3′ end of tRNA in the P site.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00255325
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