ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
The gene coding for saccharopine reductase (E.C. 1.5.1.10), an enzyme of the α-aminoadipic pathway of lysine biosynthesis in the pathogenic fungus Magnaporthe grisea, was cloned and expressed in Escherichia coli. The purified enzyme was crystallized in space groups C2 and C2221 using ammonium sulfate pH 4.8 or PEG 6000 pH 4.1 as precipitants. The unit-cell parameters are a = 115.0, b = 56.6, c = 74.3 Å, β = 111.1° for space group C2, and a = 89.3, b = 119.0, c = 195.9 Å for space group C2221. The crystals diffract to resolutions of 2.0 Å (C2) and 2.4 Å (C2221) at synchrotron sources.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444900003735
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