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  • 1
    Electronic Resource
    Electronic Resource
    Field Performance of Cell Suspension-Derived Tall Fescue Regenerants and Their Half-Sib Families (1999)
    Springer
    Crop science 39 (1999), S. 375-381 
    Details
    ISSN: 1435-0653
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Festuca arundinacea Schreb.) cv Tacuabé and their progenies were investigated in a field experiment located in Eschikon near Zürich (Switzerland). Thereby, the following two aspects were focused on. First, do regenerated plants and their progenies perform similar to seed-grown plants; and second, do different regeneration systems have an effect on the performance of the regenerants? Two sets of plants, regenerated from different single-genotype derived embryogenic suspension cultures (ESC) of tall fescue, were evaluated for agronomic traits in a replicated field experiment for three growing seasons. Embryogenic suspension cultures were either routinely subcultured or cryopreserved and re-established. Seed from the primary regenerated and seed-grown plants were harvested to evaluate morphological and phenological traits of corresponding half-sib progenies in a further field experiment. When compared with seed-grown plants of the same cultivar, primary regenerants showed reduced vegetative growth and fertility. The performance of plants regenerated from cryopreserved-reestablished ESC was not inferior to corresponding plants regenerated from routinely subcultured ESC. The performance of progenies of regenerated plants did not differ from those of seed-grown plants and was within the range of the cultivar. There was no effect of the regeneration system on the performance of corresponding progenies; thus suggesting that the weak growth of the primary regenerants was most likely of epigenetic origin. Therefore regenerated plants can be integrated into breeding programs without major restrictions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic variability of forage grass cultivars: A comparison of Festuca pratensis Huds., Lolium perenne L., and Dactylis glomerata L. (1999)
    Springer
    Euphytica 106 (1999), S. 261-270 
    Details
    ISSN: 1573-5060
    Keywords: Dactylis glomerata L. ; Festuca pratensis Huds. ; genetic variability ; Lolium perenne L. ; RAPD markers ; vegetative growth traits
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Three widely used cultivars of each of the species Festuca pratensis Huds., Lolium perenne L., and Dactylis glomerata L. were investigated by means of randomly amplified polymorphic DNA (RAPD) markers and vegetative growth traits in order to investigate genetic variability within each cultivar and to compare the level of diversity among cultivars and species. RAPD markers allowed a clear separation of the three species. Genetic variability based on RAPD markers was considerably lower for F. pratensis cultivars than for L. perenne and D. glomerata cultivars which showed similar levels of variability. The proportion of variability due to variation within cultivars, determined by an analysis of molecular variance, was lower in F. pratensis (64.6%) than in L. perenne (82.4%) and D. glomerata (85.1%). A comparison of F. pratensis and L. perenne, based on vegetative growth traits, confirmed the differences in genetic variability within cultivars. F. pratensis showed lower coefficients of genetic variation for eight of ten traits when compared to L. perenne. This study demonstrates considerable differences in genetic variability which may have consequences for the adaptability and persistency of individual cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003598705582
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