Publication Date:
2011-11-18
Description:
Abstract 2320 Transfusion-related acute lung injury (TRALI) remains a leading cause of transfusion-related death. With a decrease in antibody-mediated, plasma-induced TRALI, packed red blood cells (PRBCs) have become implicated more often. We have developed 3 experimental filters that concomitantly remove leukocytes, platelets, antibodies (abs), and lipids from PRBCs. We hypothesize that these filters remove antibodies against HLA class I and class II antigens and lipids without affecting the quality of stored PRBCs. Methods: 31 female donors whose plasma was historically positive for HLA by both Luminex™ beads (flow cytometry) and ELISA (GTI) testing were recruited and 4 male controls per filter were also drawn. Whole blood was drawn, PRBCs separated, then filtered with one of the experimental filters (B (n=11), P (n=10), or M (n=10)) or the FDA-approved Pall BPF4. Samples were drawn prior to filtration (Pre) or immediately after (Post) and at days 21 and 42 of storage. IgG, IgM, and HLA abs (by both flow cytometry and ELISA) were measured, plus a CBC was performed, pre-& post-filtration. Adenosine triphosphate (ATP), 2,3-diphoshoglycerate (2,3-DPG), pH, and neutrophil (PMN) priming activity were performed on all samples with the latter completed as the augmentation of the maximal rate of fMLP-activated PMNs measured as the superoxide dismutase inhibitable reduction of cytochrome c (nmol O2−/min). Statistical differences were determined by independent or paired (priming) ANOVA, and the data is expressed as the mean±SEM with *=p
Print ISSN:
0006-4971
Electronic ISSN:
1528-0020
Topics:
Biology
,
Medicine
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