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  • 1
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    Evaluation of the immunity factors (THC, TPP, PO, SOD, POD) of shrimp fed with the yeast Saccharomyces cerevisiae compared to shrimp fed without yeast (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Effects of S. cerevisiae on immune parameters and the WSSV resistance of the L. vannamei after 10 days of S. cerevisiae feeding were evaluated in this study. The experimental diet has the commercial shrimp composition, but 2 g of S. cerevisiae substituted 2 g of fish meal. Six hundred of subadult shrimp (10±1.02 g) were collected and divided to four groups; the 1st group (T1) and the 2nd group (T2) fed experimental diet for 10 days and T2 after 10 days injected with WSSV, whereas the 3ed group (T3) and 4th group (T4) fed with commercial diet for 10 days as control negative and control positive and T4 after 10 days injected with WSSV and all group maintained for 25 days. The immune parameters such as total hemocyte count (THC), total plasma protein (TPP), phenoloxidase activity (PO), superoxide dismutase (SOD) activity, peroxidase (POD) activity were measured. Shrimp immune parameter in T1 and T2 was significantly increased comparing to T3 and T4, but in T2 the immune response decreased in contrast to T1. The immune parameter in T4 also significantly decreased in contrast to T3. Survival rate in T1 and T3 was higher and significantly different (P〈0.05) with T2 and T4, whereas, T2 showed significantly different (P〈0.05) survival rate with T4 at the end of experiment. In conclusion these results suggest that the increased survival rate and resistance of shrimp after S. cerevisiae consumption occurs through immune modifications, such as increases in THC, TPP, SOD, SOP and PO activity, against WSSV.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: S. cerevisiae ; White Spot Syndrome Virus ; L. vannamei ; Immune parameter
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 60pp.
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  • 2
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    Monitoring of fresh water fish farms in north of khouzestan province for mortality emphasis on silver carp (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25647 | 18721 | 2018-10-08 08:31:58 | 25647 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Carp fishes culture is one of the most important subsector fisheries industry in Khouzestan province. Every year in summer high mortality occurred in carp fishes farms specially in silver carp of north Khouzestan province. Inspection of silver carp for identification microorganisms agents (Bacteria, Fungi, Parasite and virus: SVC,KHV), determine total plate count, examination physic chemical factors of farms water, histopathology, identification planktons, the main objectives of this study, which was held in 2012. 180 silver carp from 5 farms (10 ponds) with high mortality in years ago were collected in north Khouzestan province and then differents parts of fish body including: intestine, gills, body surface, eyes and liver were examined. Physico chemical parameters and plankton sampled monthly. Physico chemical parameters of water were measured all over culture period for 960 times as follows: Do(4.45-8.5 ppm), BOD5(1.1-9.25 ppm), temp(19-33c), pH(6.82-8.6), total hardness(348-10053), turbidity(5-56 FTU), Amonia(0.001- 0.06 ppm) and co2( 0.88-13.2). From a total 180 fish examined, 136 (75%) showed unknown algae infestation and 126 (70%) showed parasitic (Dactylogyrus, Gyrodactylus and metacercaria Diplostomum). The results of biotic parameters in farms showed that cyanophycae (Cylindropermopsis, Merismopedia and Nitzeshia) were the most group of phytoplanktons. Histopathological studies have shown necrosis in tobules kidney, degeneration hepatocyte and hyperplasia in gill cells and also results demonstrated the mean of total plate count (0-376 CFU/ml103). 60 samples with sighn severe hemorrhagic on surface body were collected for virology studies by PCR procedure (IQ2000 kit) and have shown which 3 sample suspected to KHV but SVC was not identified.
    Keywords: Aquaculture ; Iran ; Khouzestan Province ; Silver Carp ; Water physic chemical factor ; Microorganisms ; Histopathology ; Fresh water ; Mortality ; Fisheries ; Bacteria ; Fungi ; Parasite ; Virus ; Planktons ; Dactylogyrus ; Gyrodactylus ; Cyanophycae ; Cylindropermopsis ; Nitzeshia ; PCR
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 110
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  • 3
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    Evaluation of the immunity factors (THC, TPP, PO, SOD, POD) of shrimp fed with the yeast Saccharomyces cerevisiae compared to shrimp fed without yeast (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25724 | 18721 | 2018-10-10 10:07:05 | 25724 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Effects of S. cerevisiae on immune parameters and the WSSV resistance of the L. vannamei after 10 days of S. cerevisiae feeding were evaluated in this study. The experimental diet has the commercial shrimp composition, but 2 g of S. cerevisiae substituted 2 g of fish meal. Six hundred of subadult shrimp (10±1.02 g) were collected and divided to four groups; the 1st group (T1) and the 2nd group (T2) fed experimental diet for 10 days and T2 after 10 days injected with WSSV, whereas the 3ed group (T3) and 4th group (T4) fed with commercial diet for 10 days as control negative and control positive and T4 after 10 days injected with WSSV and all group maintained for 25 days. The immune parameters such as total hemocyte count (THC), total plasma protein (TPP), phenoloxidase activity (PO), superoxide dismutase (SOD) activity, peroxidase (POD) activity were measured. Shrimp immune parameter in T1 and T2 was significantly increased comparing to T3 and T4, but in T2 the immune response decreased in contrast to T1. The immune parameter in T4 also significantly decreased in contrast to T3. Survival rate in T1 and T3 was higher and significantly different (P〈0.05) with T2 and T4, whereas, T2 showed significantly different (P〈0.05) survival rate with T4 at the end of experiment. In conclusion these results suggest that the increased survival rate and resistance of shrimp after S. cerevisiae consumption occurs through immune modifications, such as increases in THC, TPP, SOD, SOP and PO activity, against WSSV.
    Keywords: Health ; Iran ; S. cerevisiae ; White Spot Syndrome Virus ; L. vannamei ; Immune parameter
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 60
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  • 4
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    Study on possible rehabilitation and recovery of Bandar Emam Region by reducing Nickel and Iron environmental pollutant using microorganisms (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25502 | 18721 | 2018-10-05 07:27:37 | 25502 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Influx of metallic pollutants such as iron and nickel into marine environment has been increased drastically in current era. Due to its harmful effects on living organisms, the aim of current thesis was isolation and identification of microorganisms which could reduce iron and nickel. In order to isolate bacterial strains resistant to iron and nickel, deposit samples were collected from five stations in Imam Khomeini Port cultured on BHI agar medium containing 1000 ppm of iron and 50 ppm of nickel and incubated for 24 hours in 30 oC. Endurance threshold of strains isolated to different concentrations of iron (1000- 15000 ppm) and nickel (100- 25000 ppm) in BHI broth medium were evaluated, the most resistant strains were selected and MIC and MBC were determined. Growth curve of selected strains in the presence and absence of iron and nickel were assessed by turbidity method. Along with above mentioned process, removal amount of metal by living cells and dead biomasses of selected strains were assessed by atomic absorption analysis and then 16S rRNA method was used to identify and approve the selected strains. Results of the current study were demonstrated that among of 16 and 12 resistant strains to nickel and iron, two selected strains (number16 and 7) as the most resistant strains had MIC and MBC (29800 and 29700) and (26300 and 26500), respectively. It should be mentioned that iron’s MIC was not assessable. Results of atomic absorption analysis was demonstrated that the highest amount of nickel reduction in a medium with concentration of 194 ppm and 71 ppm were 16.7% and 63% after 72 hours by selected strain number 16 respectively and in a medium containing 158 ppm iron, after 50 hours 8.4% by selected strain 7, while dead biomass of strain 16 reduced nickel to the amount of 40.6% and it reduced 21% and 4% of iron in solutions with 46 ppm and 792 ppm of iron. Results of molecular analysis demonstrated that strain 16 was Bacillus subtilis. In addition, Bacillus subtilis which has been isolated in this study with ability of nickel and iron removal is an appropriate candidate to be used in aquatic environments.
    Keywords: Ecology ; Pollution ; Iran ; Bandar Emam ; Rehabilitation ; Recovery ; Nickel ; Iron ; Environmental ; Microorganisms
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 60
    Location Call Number Expected Availability
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  • 5
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    Unknown
    Study on Possible Rehabilitation and Recovery Of Bandar Emam Region by Reducing Nickel and Iron Environmental Pollutant using Microorganisms (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Influx of metallic pollutants such as iron and nickel into marine environment has been increased drastically in current era. Due to its harmful effects on living organisms, the aim of current thesis was isolation and identification of microorganisms which could reduce iron and nickel. In order to isolate bacterial strains resistant to iron and nickel, deposit samples were collected from five stations in Imam Khomeini Port cultured on BHI agar medium containing 1000 ppm of iron and 50 ppm of nickel and incubated for 24 hours in 30 oC. Endurance threshold of strains isolated to different concentrations of iron (1000- 15000 ppm) and nickel (100- 25000 ppm) in BHI broth medium were evaluated, the most resistant strains were selected and MIC and MBC were determined. Growth curve of selected strains in the presence and absence of iron and nickel were assessed by turbidity method. Along with above mentioned process, removal amount of metal by living cells and dead biomasses of selected strains were assessed by atomic absorption analysis and then 16S rRNA method was used to identify and approve the selected strains. Results of the current study were demonstrated that among of 16 and 12 resistant strains to nickel and iron, two selected strains (number16 and 7) as the most resistant strains had MIC and MBC (29800 and 29700) and (26300 and 26500), respectively. It should be mentioned that iron’s MIC was not assessable. Results of atomic absorption analysis was demonstrated that the highest amount of nickel reduction in a medium with concentration of 194 ppm and 71 ppm were 16.7% and 63% after 72 hours by selected strain number 16 respectively and in a medium containing 158 ppm iron, after 50 hours 8.4% by selected strain 7, while dead biomass of strain 16 reduced nickel to the amount of 40.6% and it reduced 21% and 4% of iron in solutions with 46 ppm and 792 ppm of iron. Results of molecular analysis demonstrated that strain 16 was Bacillus subtilis. In addition, Bacillus subtilis which has been isolated in this study with ability of nickel and iron removal is an appropriate candidate to be used in aquatic environments.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Rehabilitation ; Recovery ; Nickel ; Iron ; Environmental ; Microorganisms
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 60pp.
    Location Call Number Expected Availability
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  • 6
    facet.materialart.
    Unknown
    Monitoring of fresh water fish farms in north of khouzestan province for mortality emphasis on silver carp (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Carp fishes culture is one of the most important subsector fisheries industry in Khouzestan province. Every year in summer high mortality occurred in carp fishes farms specially in silver carp of north Khouzestan province. Inspection of silver carp for identification microorganisms agents (Bacteria, Fungi, Parasite and virus: SVC,KHV), determine total plate count, examination physic chemical factors of farms water, histopathology, identification planktons, the main objectives of this study, which was held in 2012. 180 silver carp from 5 farms (10 ponds) with high mortality in years ago were collected in north Khouzestan province and then differents parts of fish body including: intestine, gills, body surface, eyes and liver were examined. Physico chemical parameters and plankton sampled monthly. Physico chemical parameters of water were measured all over culture period for 960 times as follows: Do(4.45-8.5 ppm), BOD5(1.1-9.25 ppm), temp(19-33•c), pH(6.82-8.6), total hardness(348-10053), turbidity(5-56 FTU), Amonia(0.001- 0.06 ppm) and co2( 0.88-13.2). From a total 180 fish examined, 136 (75%) showed unknown algae infestation and 126 (70%) showed parasitic (Dactylogyrus, Gyrodactylus and metacercaria Diplostomum). The results of biotic parameters in farms showed that cyanophycae (Cylindropermopsis, Merismopedia and Nitzeshia) were the most group of phytoplanktons. Histopathological studies have shown necrosis in tobules kidney, degeneration hepatocyte and hyperplasia in gill cells and also results demonstrated the mean of total plate count (0-376 CFU/ml103). 60 samples with sighn severe hemorrhagic on surface body were collected for virology studies by PCR procedure (IQ2000 kit) and have shown which 3 sample suspected to KHV but SVC was not identified.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Silver Carp ; Water physic chemical factor ; Microorganisms ; Histopathology ; Fresh water ; Mortality ; Fisheries ; Bacteria ; Fungi ; Parasite ; Virus ; Planktons ; Dactylogyrus ; Gyrodactylus ; Cyanophycae ; Cylindropermopsis ; Nitzeshia ; PCR
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 110pp.
    Location Call Number Expected Availability
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