ISSN:
1573-6865
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary For the precise histochemical detection of lectin binding sites of glycoproteins, the results obtained by lectin-gold-silver (LT-G-S) staining methods have been systematicaly compared with those revealed by alternative techniques of lectin-peroxidase-diaminobenzidine (LT-PO-DAB) reactions in a series of organs from different mammalian species.Ricinus communis agglutinin-I and concanavalin A were the lectins used in the present study. In the tissues subjected to the LT-G-S procedures, reactive tissue structures exhibited positive reactions of varying intensities of black. The results of control staining for the LT-G-S methods substantiated the view that the reaction products demonstrated the precise lectin binding sites of glycoproteins. The staining images obtained by the LT-PO-DAB techniques were not necessarily correlated precisely with those revealed by the LT-G-S procedures, and unavoidable background staining in pale brownish shades was noted in the majority of LT-G-S negative tissue structures. In view of these results, the LT-G-S staining methods employed in the present study are believed to be a reliable technique for the precise localization of saccharide residues of glycoproteins in light microscopy.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01324078
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