ALBERT

Notes: [Auszug] The isolated guinea pig ileum was prepared as described by Paton5. Segments of ileum were suspended in a Krebs physiological buffer at 37 C, saturated with 95%O2-5%CO2. The buffer contained NaCl, 118 mM; KC1, 4.75 mM; CaCl2.2H2O, 2.54 mM; MgSO4.7H2O, 1.19mM; KH2PO4, 1.19mM; glucose, llmM; NaHCO3, ...

Notes: [Auszug] SIR-Contrary to what Hall et al.1 imply, quantitative autoradiography was developed and used to measure local rates of cerebral blood flow by Kety and co-workers2^ well before 1981. This technique has also been applied to determination of local rates of biochemical processes in vivo56. Quantitative ...

Notes: [Auszug] Experimental work on the melting relations of model perido-titic systems charged with CO2 has shown that liquids of carbo-natitic and kimberlitic composition may be produced by a small degree of partial melting of a carbonated peridotite1'2. This melting is likely to be caused by a change in the ...

Notes: [Auszug] Brain contents of 14C-dopamine and 14C-noradrenaline were determined 40 min after the injection of 14C-tyrosine (55 ug kg-1; specific activity, 410 mCi/mmol, uniformly labelled, New England Nuclear Corporation) into the tail vein of the female albino mouse (20-30 g). Maximum incorporation of ...

Notes: [Auszug] Capacitance monitoring7'8 have proven valuable for measuring surface membrane area on a millisecond timescale in adrenal chromaffin3'7'9"11 and other12"16 cells. The fluorescent styryl dye FM1-43 has been useful for optical tracking of synaptic vesicle recycling in a variety of nerve ...

Notes: Summary This article analyzes the nature of the aerosol information that current or planned spacecraft measurements could contribute toward the required input data for studies of natural anthropogenic influences on the middle atmosphere, and their consequent effects on our weather and climate. The analysis is conducted with particular reference to the solar occultation sounding technique as applied by the SAGE I experiment on the Atmospheric Explorer Mission B spacecraft. Its conclusions should prove to be of use in both the interpretation of the SAGE I data, and in the design of the follow-on mission on the Earth Radiation Budget satellite. Our analysis shows, in particular, that further studies are required in: the choice and number of sounding channels; the data taking sequence in relation to the atmospheric regions probed; the accuracy and vertical resolution of the atmospheric profiling, and their dependence on both the instrument/spacecraft parameters and the data inversion techniques; and the data reduction procedures. Neither of the selected channels is in a one-to-one relationship with an atmospheric constituent; hence, unless further assumptions are introduced, inversion techniques based on such a property are not applicable. The aerosol wavelengths are not satisfactory as they are only sensitive to the large size tail of the aerosol size distribution rather than to the predominant sizes; for these, UV wavelengths would be required. Owing to the change of the Sun's shape due to atmospheric refraction as the Sun either sets or rises, the higher altitudes will be scanned fewer times than the lower altitudes. Also, because transmission approaches rapidly unity above ∼40 km, the same high altitudes are more sensitive to measurement errors-errors that will propagate to lower altitude determinations when inverted profiles are reconstructed from the top of the atmosphere. These two factors, combined with the small air mass values at the high altitudes, are the cause of the mathematical ill-conditioning of the inversion problem. They point toward the need for a data-taking sequence strategy that would trade off between data storage and transmission constraints, larger accuracy at the high altitudes, and proper division of the atmosphere in order to overcome the ill-conditioning. Likewise, and as a result of the above considerations, there is a need for a detailed trade off study between data accuracy and vertical resolution of the reconstructed profiles. This should take into account the seasonal and geographical variations in the distribution of atmospheric constituents, as well as a representative statistical set at any given location and time, appropriate error measures and their vertical profiles, and several inversions utilizing as initial guesses profiles that depart from the true ones. It is also shown that the aerosol and ozone number densities cannot be recovered simultaneously without introducing some formula for the aerosol extincition or assumptions on the form of the aerosol size distribution. This problem is not resolved by the addition of sounding channels because each such channel introduces an additional unknown aerosol extinction. Thus, one is led to a separate rather than a simultaneous determination of the various constituents by resorting to complementary measurements. For a future experiment, it is suggested to determine the ozone separately from measurements at a close pair of appropriate wavelengths between which the aerosol extinction varies slowly whereas that of ozone exhibits a rapid variation. A similar technique could also be used for the separate determination of NO2. The relaxation-type of inversion suggest byChu andMcCormick (1979) does not seem to be appropriate because each channel is not sensitive selectively to an individual constituent, the aerosol channels are not sensitive to the important sizes in the distribution, and the sensitivity of the channels to the constituents of interest varies greatly with altitude. In the retrieval of the vertical profiles, the cause of the ill-conditioning of the inversion is identified. Two approaches are suggested for overconing this problem: (i) build the profile starting from the top of the atmosphere (forward procedure) but with an initial layer of sufficient air mass, or preferably (ii) reconstruct the profile from the lowest altitude reached (backward procedure) with a renormalization at the top of the atmosphere. In this process, the minimization search method (Fymat, 1976) would appear to be a better technique than the onion-peeling technique, as demonstrated byMill andDrayson (1978). In order to maximize the scientific return of SAGE I, a data inversion procedure is proposed. It assumes that (i) there are no aerosols above ∼25 km, and no NO2 below this altitude (as suggested byChu andMcCormick, 1979), (ii) below ∼25 km, ozone (and NO2, if present) could be determined separately, and (iii) the aerosol has a known refractive index at all wavelengths of interest, is assumed to be spherical (or describable in terms of ‘equivalent spheres’), and the minimum and maximum radii of its size distribution are known a priori. Under these assumptions, it is possible to retrieve the neutral density, NO2 and O3 profiles above ∼25 km, by either the forward or the backward procedure described above. Taking into consideration the power law variation of the air density with altitude, it is further possible to reconstruct the corresponding profiles at all the lower altitudes from the determinations in the altitude range ∼30–40 km. Below ∼25 km, the four SAGE I channels would then all become available for the aerosol inversion. While the profile reconstruction could proceed as for the higher altitudes, the aerosol inversion at each individual altitude presents problems of its own. Results of numerical experiments for aerosol inversion using all four SAGE wavelengths and seven different inversion routines are presented. If good a priori information is available on the sought size distribution solution, reasonably satisfactory inversions can be performed (see line 1 of Table 2, and Fig. 3c and 3d). However, in the absence of such information, there are as many solutions as inversion methods tried, in complete conformity with the well-known ill-conditioning of the problem. Among methods providing physically meaningful solutions, no method could be singled out as preferable to the others. In these inversions, the data were assumed to be exact, and 99% of the distribution were used. Under different conditions, the nonuniqueness of the inversion would be further compounded. Lastly, based on the present study, a strategy is suggested for the design and data interpretation of a follow-on SAGE-type experiment. Considering the important advantages to this problem presented by forward scattering, as demonstrated byFymat andMease (1978), a composite (extinction-forward scattering) experiment is recommended for the future experiments.

Notes: Abstract Evidence relating to the existence of crystalline bone mineral in vivo is considered, and bone apatite crystal structure investigated using an x-ray powder diffraction technique. Specimens of femoral compacta excised post-mortem from male and female subjects ranging from 3 1/2 years to 87 years of age have been studied. Values of the ratio c/a of bone apatite crystal cell axes for females are significantly higher (p〈0.05) than for males. Moreover, significant change of c/a with age is observed for males (p=0.0005) but not for females (p=0.30). Differences in c/a are interpreted as indicating substitution of constituent ions in the bone apatite crystals.