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  • 1
    Electronic Resource
    Electronic Resource
    The pssB gene product of Rhizobium leguminosarum bv. trifolii is homologous to a family of inositol monophosphatases (1999)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 173 (1999), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The Rhizobium leguminosarum bv. trifolii region encoding pssA and pssB genes was cloned. The pssB gene located upstream of the pssA encoded a 28.36-kDa protein which displayed 97.5% identity with the PssB of R. leguminosarum bv. viciae. Inactivation of the pssB gene by insertion of the lacZ-Gmr cassette resulted in the significant increased production of exopolysaccharide in comparison to the wild-type level. A mutant strain was also defective in nitrogen fixation suggesting a regulatory role of pssB in symbiosis with clover.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1999.tb13520.x
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  • 2
    Electronic Resource
    Electronic Resource
    Rhizobial siderophore as an iron source for clover (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 85 (1992), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Iron uptake by clover plants (Trifoliuin pratense L. cv. Hruszowska) was studied using radioactive ferric iron (55FeCl2). As shown by autoradiography of non-infected plants, purified rhizobial siderophore isolated from Rhizobium leguminosarum by, trifolii, stimulated the uptake and shoot transport of iron. Addition of rhizobial siderophore into the growth medium of nodulated clover did not affect the iron transport to the shoots. In the absence of the rhizobial siderophore, clover infected by either nitrogen-fixing (Nod− Fix+) or nonfixing (Nod+ Fix−) R. L. trifolii strains took up and transported into the shoots more iron than the non-infected control plants. Nodulated clover reduced Fe(III) more efficiently than the non-infected control plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1992.tb05825.x
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  • 3
    Electronic Resource
    Electronic Resource
    Rhizobium trifolii mutants deficient in exopolysaccharide production (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 66 (1986), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Spontaneous mutants of Rhizobium trifolii 24AR5 which did not produce exopoly-saccharide were isolated. The non-mucoid mutants formed small white and ineffective nodules on both red and white clover. These nodules contained infection threads, but only a small number of bacteria were released into nodule cells, and bacteroids were rarely observed. The non-mucoid phenotype was not complemented by the symbiotic plasmid (pJB5JI) of Rhizobium leguminosarum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1986.tb05602.x
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  • 4
    Electronic Resource
    Electronic Resource
    Role of noncarbohydrate substitutions of Rhizobium exopolysaccharide in nodulation process (1985)
    Springer
    Archives of microbiology 143 (1985), S. 307-310 
    Details
    ISSN: 1432-072X
    Keywords: Exopolysaccharides ; Nodulation ; Rhizobium trifolii ; O-acetyl groups ; Pyruvic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Exopolysaccharides (EPS) of nodulating strains of Rhizobium trifolii and Rhizobium leguminosarum added to red clover seedlings before inoculation reduced the number of nodules. The inhibition of the nodulation was correlated with the amount of EPS. The preparations of EPS from mutants defective in early stages of nodulation (Roa- or Hac-) did not affect the nodulation, whereas EPS from mutants deficient in late stages (post Hac-) exerted an inhibitory effect. Inactive preparation of EPS contained less O-acetyl groups and pyruvic acid residues. Deacetylation and depyruvylation of EPS from R. trifolii Nod+ abolished it inhibitory effect. It was concluded that noncarbohydrate substitutions (acetate, pyruvate) are involved in EPS effect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00411255
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  • 5
    Electronic Resource
    Electronic Resource
    Detection of small cryptic plasmids in Salmonella typhimurium strain LT2 (1978)
    Springer
    Archives of microbiology 119 (1978), S. 87-90 
    Details
    ISSN: 1432-072X
    Keywords: Salmonella typhimurium strain LT2 (ColIb) ; Cryptic plasmids ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Small cryptic plasmids of molecular weights ranging from 1 to 3 Mdal were detected by electron microscopy in Salmonella typhimurium strain LT2 (ColIb). They were divided into different size classes. Two of the cryptic plasmids were transferred simultaneously with ColIb to Escherichia coli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00407933
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  • 6
    Electronic Resource
    Electronic Resource
    Nif plasmid from Lignobacter (1981)
    Springer
    Archives of microbiology 130 (1981), S. 322-324 
    Details
    ISSN: 1432-072X
    Keywords: Lignobacter ; Salmonella typhimurium ; Escherichia coli ; Nif plasmid ; Tn9 ; Transduction ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lignobacter strain K17 is able to degrade aromatic compounds and to fix atmospheric nitrogen. It was proved that capacity for nitrogen fixation by Lignobacter was plasmid mediated. Plasmid pUCS100 (17.5 Mdal) carrying nif genes was transferred from Lignobacter to Escherichia coli SK1592 and Salmonella typhimurium. The transposon Tn9 was translocated to pUCS100 to facilitate selection of Nif+ bacteria. E. coli SK1592 harboring the new plasmid (pUCS101) reduced acetylene under anaerobic conditions. Plasmids pUCS100 and pUCS101 were not stably maintained in E. coli and S. typhimurium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425948
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  • 7
    Electronic Resource
    Electronic Resource
    Siderophore production and utilization byRhizobium trifolii (1989)
    Springer
    BioMetals 2 (1989), S. 45-49 
    Details
    ISSN: 1572-8773
    Keywords: Siderophore production ; Siderophore utilization ; Rhizobium trifolii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Several strains ofRhizobium trifolii were tested for their ability to synthesize and utilize phenolate or hydroxamate types of siderophores. None of the nodulating strains ofR. trifolii was able to produce detectable amounts of siderophores. Only the non-nodulating strainR. trifolii AR6 formed a phenolate siderophore, which stimulated the growth of the siderophore-negative mutant AR65. Other strains ofR. trifolii could not utilize iron from exogenously supplied Desferal, pseudobactin or citrate. The siderophore fromR. trifolii AR6 and 2,3-dihydroxybenzoic acid slightly stimulated the growth of someR. trifolii strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01116201
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  • 8
    Electronic Resource
    Electronic Resource
    Infection of clover by plant growth promoting Pseudomonas fluorescens strain 267 and Rhizobium leguminosarum bv. trifolii studied by mTn5-gusA (2000)
    Springer
    Antonie van Leeuwenhoek 78 (2000), S. 1-11 
    Details
    ISSN: 1572-9699
    Keywords: clover colonization ; endophytic bacteria ; GUS-marker ; Pseudomonas fluorescens ; Rhizobium leguminosarum bv. trifolii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plant growth promoting Pseudomonas fluorescens strain 267, isolated from soil, produced pseudobactin A, 7-sulfonic acid derivatives of pseudobactin A and several B group vitamins. In coinoculation with Rhizobium leguminosarum bv. trifolii strain 24.1, strain 267 promoted clover growth and enhanced symbiotic nitrogen fixation under controlled conditions. To better understand the beneficial effect of P. fluorescens 267 on clover inoculated with rhizobia, the colonization of clover roots by mTn5-gusA marked bacteria was studied in single and mixed infections under controlled conditions. Histochemical assays combined with light and electron microscopy showed that P. fluorescens 267.4 (i) efficiently colonized clover root surface; (ii) was heterogeneously distributed along the roots without the preference to defined root zone; (iii) formed microcolonies on the surface of clover root epidermis; (iv) penetrated the first layer of the primary root cortex parenchyma and (v) colonized endophytically the inner root tissues of clover.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002619824691
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  • 9
    Electronic Resource
    Electronic Resource
    Tn5 insertion mutants of Pseudomonas sp. 267 defective in siderophore production and their effect on clover (Trifolium pratense) nodulated with Rhizobium leguminosarum bv. trifolii (1996)
    Springer
    Plant and soil 179 (1996), S. 269-274 
    Details
    ISSN: 1573-5036
    Keywords: plant growth promotion ; Pseudomonas sp. ; Rhizobium leguminosarum bv. trifolii ; siderophore ; Tn5 mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Pseudomonas sp. strain 267 isolated from soil promoted growth of different plants under field conditions and enhanced symbiotic nitrogen fixation in clover under gnotobiotic conditions. This strain produced pyoverdine-like compound under low-iron conditions and secreted vitamins of the B group. The role of fluorescent siderophore production in the beneficial effect of strain 267 on nodulated clover plants was investigated. Several non-fluorescent (Pvd-) Tn5 insertion mutants of Pseudomonas sp. strain 267 were isolated and characterized. The presence of Tn5 insertions was confirmed by Southern analysis of EcoRI digested genomic DNA of each derivative strain. The siderophore-negative mutants were compared to the parental strain with respect to their growth promotion of nodulated clover infected with Rhizobium leguminosarum bv. trifolii 24.1. We found that all isolated Pvd- mutants stimulated growth of nodulated clover plants in a similar manner to the parental strain. No consistent differences were observed between strain 267 and Pvd- derivatives strains with respect to their plant growth promotion activity under gnotobiotic conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00009337
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  • 10
    Electronic Resource
    Electronic Resource
    Enhancement of symbiotic nitrogen fixation by vitamin-secreting fluorescent Pseudomonas (1993)
    Springer
    Plant and soil 154 (1993), S. 211-217 
    Details
    ISSN: 1573-5036
    Keywords: nitrogen fixation ; plant growth promotion ; Pseudomonas sp. ; Rhizobium leguminosarum ; vitamins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Fluorescent Pseudomonas sp. strain 267 promotes growth of nodulated clover plants under gnotobiotic conditions. In the growth conditions (60 μM FeCl3), the production of siderophores of the pseudobactin-pyoverdin group was repressed. Plant growth enhancement results from secretion of B vitamins by Pseudomonas sp. strain 267. This was proven by stimulation of clover growth by naturally auxotrophic strains of Rhizobium leguminosarum bv. trifolii and marker strains E. coli thi- and R. meliloti pan- in the presence of the supernatant of Pseudomonas sp. strain 267. The addition of vitamins to the plant medium increased symbiotic nitrogen fixation by the clover plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00012526
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