ISSN:
1573-0603
Keywords:
tissue culture
;
monocyte-macrophages
;
Plastek-C
;
polystyrene
;
cell growth
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Many investigators have found that the surface on which cells are cultured can be an important factor in their adherence and subsequent growth. To enhance the development of monocyte-macrophage cultures, we have grown these cells on polystyrene tissue cultureware that has been treated with gas plasma to induce a positively charged culture surface (Plastek-C cultureware from Tekmat). Human monocyte-macrophages grown on Plastek-C tissue cultureware demonstrated a greater cell protein content during culture development as compared to monocyte-macrophages grown on standard polystyrene tissue cultureware. Part of the reason for enhanced development of human monocyte-macrophage cultures on Plastek-C cultureware seemed to be due to a higher density of cells maintained during the initial stages of culture development.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01409100
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