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Mass transfer parameters in an immobilized glucoamylase column by pulse response analysis (1983)

Sirotti, David A. ; Emery, Alden

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 25 (1983), S. 1773-1779

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ISSN: 0006-3592

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Pulses of several nonreacting solutes were fed to a column containing immobilized enzyme catalyst beads. The response curves were recorded, and their first and second moments were determined by numerical integration. From the moment analysis, the void volume, internal porosity, and tortuosity of the support were calculated.

Additional Material: 2 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bit.260250707

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Immobilization of glucose isomerase to cellulose (1978)

Sirotti, David A. ; Emery, Alden

Hoboken, NJ : Wiley-Blackwell

AIChE Journal 24 (1978), S. 1040-1046

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ISSN: 0001-1541

Keywords: Chemistry ; Chemical Engineering

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Glucose isomerase was covalently bonded to Solka-floc and DEAE cellulose by several techniques. The best results were obtained with techniques that opened the crystalline structure of the cellulose and kept it that way during immobilization. The most active catalyst was prepared by mercerizing Solka-floc, cross-linking it in that expanded configuration with epoxides, and immobilizing the enzyme with cyanogen bromide. The second most active catalyst was made using DEAE cellulose and a mixture of di and tri-epoxides as immobilization reagent.The practical consequences of the results were tested by designing reactors to produce high fructose syrup using the activity and stability of several of the preparations. Since the activity decays with time, equations were developed to give the optimum time of running before the catalyst was discarded. The most promising one was the second most active; the most active catalyst yielded a production cost three times as great because of the large quantities of cyanogen bromide needed.

Additional Material: 5 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/aic.690240615

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