ISSN:
1432-5225
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Summary A simple automated analytical method for separation and quantitative determination of sugars from acidic and enzymatic hydrolysates of wood polysaccharides via borate complex ion exchange chromatography is described. Samples are analysed without any interferring manipulation like derivatization, deionization or deproteinization, and even sulphuric acid hydrolysates can be directly applied to the column. As little as 0.5 μg of an individual sugar can be quantitatively measured. Examples of the separation of common monomeric sugars and mixtures of mono-and disaccharides and uronic acids are given.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00353480
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