ISSN:
1573-4978
Keywords:
Drosophila
;
20-hydroxyecdysone
;
puromycin
;
protein synthesis
;
cycloheximide
;
imaginal wing disc
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The effect of cycloheximide and puromycin on 20-hydroxyecdysone-induced protein synthesis in wing discs of Drosophila melanogaster has been studied by one-dimensional and two-dimensional SDS polyacrylamide electrophoresis. It is found that puromycin, but not cycloheximide, when applied simultaneously with the hormone enhanced the hormone-induced synthesis of the ‘early’ and ‘late’ proteins. However, when puromycin was applied after hormone treatment, only the ‘late’ proteins were induced. The possible implication of these observations is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00368874
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