ISSN:
1617-4623
Keywords:
Key words Restriction enzyme-mediated insertion (REMI)
;
Promoter-tagged REMI
;
Promoter tagging
;
Gene tagging
;
Aspergillus niger
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Promoter-tagged restriction enzyme-mediated insertion (PT-REMI) mutagenesis was performed in the fungus Aspergillus niger, using a plasmid containing a strong transcriptional promoter. Two DNA-tagged mutants were analyzed in detail. A white-spored mutant was shown to contain a plasmid insertion that disrupted a gene that shows a high degree of homology to the polyketide synthase gene wA of A. nidulans. A morphological mutant was shown to contain a plasmid insertion in the 5′ upstream region of a gene that strongly resembles COX5, which encodes the cytochrome c oxidase subunit V. Insertion of the plasmid resulted in enhanced expression of the COX5 RNA, demonstrating that the combination of REMI with a promoter-containing insert can be used to activate gene transcription.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/PL00008638
Permalink