Publication Date:
2013-10-02
Description:
Background: Diacylglycerol kinase (DGK) is a key enzyme that regulates diacylglycerol (DG) turnover and is involved in a variety of physiological functions. The isoform DGKtheta has a unique domain structure and is the sole member of type V DGK. To reveal the spatial and temporal expression of DGKtheta we performed immunohistochemical staining on paraffin sections of mouse embryos. Results: At an early stage of development (E10.5 and 11.5), the expression of DGKtheta was prominently detected in the brain, spinal cord, dorsal root ganglion, and limb bud, and was also moderately detected in the bulbus cordis and the primordium of the liver and gut. At later stages (E12.5 and 14.5), DGKtheta expression persisted or increased in the neocortex, epithalamus, hypothalamus, medulla oblongata, and pons. DGKtheta was also evident in the epidermis, and nearly all epithelia of the oropharyngeal membrane, digestive tract, and bronchea. At prenatal developmental stages (E16.5 and E18.5), the expression pattern of DGKtheta was maintained in the central nervous system, intestine, and kidney, but was attenuated in the differentiated epidermis. Conclusion: These results suggest that DGKtheta may play important physiological roles not only in the brain, but also in diverse organs and tissues during the embryonic stages.
Electronic ISSN:
1471-213X
Topics:
Biology
,
Medicine
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