ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
A novel collagen sponge that can protect cell leakage during cell seeding was developedby wrapping all the surfaces except the upside of a collagen sponge with membrane that has poressmaller than cell. The collagen sponge was used for three-dimensional culture of human bonemarrow-derived mesenchymal stem cells (MSCs). The cells adhered to the collagen, andproliferated to fill the spaces in the sponge. The cell seeding efficiency was higher than 95%. TheMSCs cultured in the collagen sponge in the chondrogenic induction medium supplemented withTGF-β3 and BMP6 expressed genes encoding type II collagen, SOX9 and aggrecan. HE stainingindicated the round morphology of differentiated cells and the extracelluler matrices werepositively stained by safranin O and toluidine blue. Type II collagen and cartilage proteoglycanwere detected by immunostaining with anti-type II collagen and anti-cartilage proteoglycan. Theseresults suggest the chondrogenic differentiation of MSCs. The collagen sponge facilitated cellseeding and chondrogenic differentiation of MSCs, and will be useful for cartilage tissueengineering
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/53/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.1101.pdf
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