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  • 1
    Electronic Resource
    Electronic Resource
    RICE AS A MODEL FOR COMPARATIVE GENOMICS OF PLANTS (2002)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Plant Physiology and Plant Molecular Biology 53 (2002), S. 399-419 
    Details
    ISSN: 1040-2519
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Notes: Abstract Rapid progress in rice genomics is making it possible to undertake detailed structural and functional comparisons of genes involved in various biological processes among rice and other plant species, such as Arabidopsis. In this review, we summarize the current status of rice genomics. We then select two important areas of research, reproductive development and defense signaling, and compare the functions of rice and orthologous genes in other species involved in these processes. The analysis revealed that apparently orthologous genes can also display divergent functions. Changes in functions and regulation of orthologous genes may represent a basis for diversity among plant species. Such comparative genomics in other plant species will provide important information for future work on the evolution of higher plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.arplant.53.092401.134447
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation and characterization of aminopterin-resistant cell lines in maize (1981)
    Springer
    Planta 153 (1981), S. 436-442 
    Details
    ISSN: 1432-2048
    Keywords: Aminopterin-resistant variants ; Cell culture ; Folate analog ; Variant selection ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Aminopterin-resistant cell lines of maize were isolated by two different procedures of callus selection and by plating suspension cultures on drugcontaining medium after mutagen treatment. Efficiencies of different methods of variant selection were compared. Four aminopterin-resistant cell lines were shown to be 10–40 times more resistant than the parental cell line, and they were also resistant to another folate analog, methotrexate. The results suggest that alterations in at least three different cell properties could be responsible for resistance; 1) increased dihydrofolate reductase activity, 2) altered aminopterin sensitivity of dihydrofolate reductase, and 3) reduced drug uptake. One of the resistant cell lines showed more than one alteration, but its resistance proved to be unstable. The results suggest that stable changes which may or may not be of genetic origin and also unstable physiological changes or a combination of both could lead to aminopterin resistance in maize cell cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00394982
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation of biochemical mutants using haploid mesophyll protoplasts ofHyoscyamus muticus (1983)
    Springer
    Planta 159 (1983), S. 18-24 
    Details
    ISSN: 1432-2048
    Keywords: Amino acid (Hyoscyamus mutant) ; Auxotroph ; Crossfeeding (genetic) ; Hyoscyamus ; Mutant (Hyoscyamus) ; Reversion (genetic) ; Somatic complementation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two amino-acid auxotrophic cell clones ofHyoscyamus muticus, VA5 (His-) and VIIIB9 (Trp-), isolated in a previous experiment have been characterised quantitatively. Studies of growth in the presence and absence of histidine and tryptophan and an examination of dose-response relationships for the two amino acids confirmed the strict auxotrophy of both cell lines. No revertants to prototrophy were detected in either cell line after more than two years in culture. N-methyl-N′-nitro-nitrosoguanidine did not induce reversion in VA5 cell populations. Wild-type aggregates mixed in various combinations with VA5 and VIIIB9 cells could be recovered after plating in selective conditions. No cross-feeding was detected, either between wild-type and auxotrophic cells or between the auxotrophic lines themselves. Both variants were recloned by protoplast culture. All protoplast-derived clones were auxotrophic. The auxotrophic phenotypes behaved as recessive traits in protoplast-fusion experiments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00998809
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  • 4
    Electronic Resource
    Electronic Resource
    Production of Cytoplasmic Male Sterile Rice ( Oryza Sativa L.) by Cell Fusion (1989)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 7 (1989), S. 1171-1174 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] In order to transfer cytoplasmic male sterility (CMS) of an indica rice, Chinsurah Boro II (CBII), to a major japonica variety, Nipponbare, iodoacetamide inactivated Nipponbare protoplasts were fused with X-irradiated CBII protoplasts by electrofusion. Among 16 fusion-derived plants having diploid ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt1189-1171
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  • 5
    Electronic Resource
    Electronic Resource
    Picking genes in the rice genome (2002)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 20 (2002), S. 983-984 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Despite numerous attempts in several model species, gene targeting by homologous recombination has remained an elusive goal in plant research. In this issue, Iida et al. combine refinements in A. tumefaciens–mediated transformation, a strong negative selection protocol, and stringent PCR ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt1002-983
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  • 6
    Electronic Resource
    Electronic Resource
    Insect Resistant Rice Generated by Introduction of a Modified δ-endotoxin Gene of Bacillus thuringiensis (1993)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 11 (1993), S. 1151-1155 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] As a first step towards development of insect resistant rice we have introduced a truncated δ-endotoxin gene, cryIA(b) of Bacillus thuringiensis (B.t.) which has specific biological activity against lepidopteran insects into a japonica rice. To highly express the cryIA(b) gene in rice the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt1093-1151
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  • 7
    Electronic Resource
    Electronic Resource
    Negative fallout from public sentiment in Japan (2004)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 22 (2004), S. 943-943 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] To the editor: As concerned plant scientists at major plant science research institutions in Japan, we would like to express our collective concern over the impact of Japanese public resistance to plant genetic engineering on the actions of local and national government. We are concerned that ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0804-943a
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  • 8
    Electronic Resource
    Electronic Resource
    Fertile transgenic rice plants regenerated from transformed protoplasts (1989)
    [s.l.] : Nature Publishing Group
    Nature 338 (1989), S. 274-276 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The kanamycin resistance (Kmr) gene coding for neomycin phosphotransferase II (NPT II) is often used as a selectable marker to produce transgenic plants1. We found, however, that the bacterial hph gene is a more effective marker for the selection of stable transformants from embryogenic rice ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/338274a0
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  • 9
    Electronic Resource
    Electronic Resource
    Adaptation of photoperiodic control pathways produces short-day flowering in rice (2003)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 422 (2003), S. 719-722 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The photoperiodic control of flowering is one of the important developmental processes of plants because it is directly related to successful reproduction. Although the molecular genetic analysis of Arabidopsis thaliana, a long-day (LD) plant, has provided models to explain the control of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature01549
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  • 10
    Electronic Resource
    Electronic Resource
    Wheat protoplast culture: embryogenic colony formation from protoplasts (1988)
    Springer
    Plant cell reports 7 (1988), S. 414-417 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Wheat (Triticum aestivum L. cv Chinese Spring) protoplasts were isolated from immature embryos or embryogenic calli (3–4 weeks of culture on MS medium with 32 mg/1 dicamba) and cultured in R2 medium containing 2 mg/1 2,4-D by the nurse culture methods originally developed for rice protoplasts (Kyozuka et al. 1987). Protoplasts isolated from embryogenic calli started to divide within 3–5 days and formed colonies at frequencies up to 2% after 3–4 weeks of culture, while protoplasts isolated from immature embryos formed colonies at much lower frequency (less than 0.1%). Some of these colonies were “embryogenic”, and they appeared at a frequency of approximately 0.5% of colonies formed when callus-derived protoplasts were used. From two of those “embryogenic” colonies, calli were regenerated and albino shoots and roots were obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269526
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