ISSN:
1573-5095
Keywords:
in vitro propagation
;
organogenesis
;
plantlet regeneration
;
rooting
;
Western hemlock
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract An in vitro propagation protocol is described for western hemlock, an important forestry species in Canada. For shoot bud induction, embryonic explants were placed initially on one-third strength Schenk and Hildebrandt medium containing 5 μM N6-benzyladenine (BA) or 5 μM BA in combination with either 5 μM kinetin or 5 μM 2-isopentenyl adenine for 14 days. The explants were transferred to basal medium without cytokinins for 3 weeks, and then to basal medium containing 0.05% activated charcoal. Elongating shoots were subcultured every 4 weeks on charcoal medium. Shoots, 10 mm in stem height, were rooted either in agar or sterilized peat/perlite (1:1). Up to 70% of the shoots formed roots when they were transferred to the latter, moistened with 1/2 strength Gresshoff and Doy medium containing 5 μM α-naphthaleneacetic acid. About 90% of the plantlets survived transfer to greenhouse conditions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00034127
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