ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Fibrin is a natural substrate for growth, adhesion, and migration of mature endothelialcells (ECs) and a candidate coating material in approaches to graft endothelialization. Adiposetissue represents an abundant, practical source of donor tissue for stem cells which may be a usefulsource for engineering of vascular grafts. However, the optimal substrates that promotedifferentiation of adipose tissue-derived stem cells (ASCs) into ECs remain to be elucidated. In thepresent study, we investigated whether fibrin can be used as a substratum to support in vitro ECsdifferentiation of ASCs and whether fibrinogen concentration can be affect on ECs differentiation ofASCs. For determination of phenotypic characteristics of ASCs used in this experiment, weperformed flow cytometry analysis. ASCs were plated on fibrin composed of varyingconcentrations of fibrinogen and induced into ECs differentiation in presence of VEGF. Beforeinducing into ECs, ASCs did not express any markers of hematopoietic cells (CD34, CD45), ECs(CD31, CD34), and endothelial progenitor cells (CD34, CD133, Flk-1). The degree of ECsdifferentiation was determined by capillary network formation, ECs-specific gene expression, andF-actin assembly. During the first 12 h after seeding, cells spread randomly, moved and formedsmall interconnected clusters. These clusters decreased in size and formed a capillary tube at 48 h.During the further incubation in presence of VEGF for 7 days, ASCs expressed mRNA and proteinof von Willebrand factor (vWF). The degree of ECs differentiation of ASCs was consistentlydecreased as fibrinogen concentration increase. Fibrin may be used as biomatrix to promotedifferentiation of ASCs into ECs for tissue engineering
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.17.pdf
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