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  • 1
    Electronic Resource
    Electronic Resource
    Photosynthetic studies with mesophyll protoplasts from Notonia grandiflora, a crassulacean acid metabolism plant (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 48 (1980), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A method is described for rapid enzymatic isolation of mesophyll protoplasts and cells from the crassulacean acid metabolism (CAM) plant Notonia grandiflora DC. The mesophyll protoplasts exhibited high rates of 14CO2 fixation both in the light (45 μmol of CO2 fixed mg−1 Chl h−1) and in the dark (20 μmol of CO2 fixed mg−1 Chl h−1). The protoplasts also showed O2 evolution (40 μmol of O2 evolved mg−1 Chl h−1) without added bicarbonate. Exogenously added bicarbonate had no stimulating effect on the O2 evolution. Analyses of early photosynthetic products in the light showed the formation of both C3 and C4 acids. Aspartate was found to be a predominant photosynthate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1980.tb03276.x
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  • 2
    Electronic Resource
    Electronic Resource
    Photosynthesis in cell suspension cultures of the CAM plant Chamaecereus sylvestrii (Cactaceae) (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 49 (1980), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Photosynthetic properties of cell suspension cultures derived from the callus proliferation of cladophyll explants of Chamaecereus sylvestrii Spegazzini were studied. High content of chlorophyll (105–120 μg/g fresh weight), cyanide sensitive O2 uptake and maximal rates of O2 evolution (100–115 μmol/mg Chl x h) and CO2 fixation (130–150 μmol/mg Chl x h) were some of the properties of the exponential phase cells. Determination of the component reactions, viz. photosystems I and II and photophosphorylation of the chloroplasts isolated from the cells, indicated normal development and functioning of the photosynthetic machinery.Studies on the enzymatic reactions as well as the determination of the early products of 14CO2 fixation in light in these cells implicated the operation of both autotrophic and non-auto-trophic pathways, the latter being less pronounced. The diurnal oscillation of titratable acidity and malate content found in the intact cladophyll tissues was absent in the cultured cells. Evidences for a rapid and continuous drain of carbon from malate into the citrate and isocitrate components of the TCA cycle via pyruvate after decarboxylation, and then into the amino acid pool are presented. The absence of large vacuoles and the rapid turnover of malate are considered to account for the lack of diurnal fluctuation of organic acide in the cell cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1980.tb03336.x
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  • 3
    Electronic Resource
    Electronic Resource
    The isolation, culture and organ differentiation from mesophyll protoplasts ofMollugo nudicaulis Lam., a C4 species (1980)
    Springer
    Cellular and molecular life sciences 36 (1980), S. 60-61 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Isolation and culture of mesophyll protoplasts fromMollugo nudicaulis, a C4 species, is reported. Protoplasts developed into callus with root formation. However, no shoot was differentiated. This work was carried out as a part of our overall objective of inducing somatic fusion between the protoplasts of C3 and C4 species ofMollugo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02003972
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  • 4
    Electronic Resource
    Electronic Resource
    Citrinin, a phytotoxin? (1975)
    Springer
    Cellular and molecular life sciences 31 (1975), S. 1415-1417 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Observations from preliminary experiments to discover the phytotoxicity, if any, of the fungal metabolite, citrinin, are presented. There seems to be a positive indication, warranting further investigation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01923219
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  • 5
    Electronic Resource
    Electronic Resource
    Mechanical separation of palisade and spongy-parenchyma cells from the leaves of mesomorphic dicotyledons for photosynthetic studies (1983)
    Springer
    Planta 157 (1983), S. 105-110 
    Details
    ISSN: 1432-2048
    Keywords: Mesophyll, cell separation ; Palisade cells ; Photosynthesis in different cells ; Ribulose-1,5-bisphosphate carboxylase ; Spongy parenchyma ; Zinnia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Palisade and spongy-parenchyma cells were isolated from the leaves of a number of mesomorphic dicotyledons by simply brushing the upper and lower sides, respectively, of the leaves with nylon brushes. Cross-contamination with the opposite cell type was minimal and both cell types were photosynthetically as active as leaf discs. The rates and early products of CO2 incorporation in the two cell types isolated from Zinnia elegans Jacq. plants grown in full natural light were the same, indicating that the photosynthetic physiology of the two cell types is quite similar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00393643
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  • 6
    Electronic Resource
    Electronic Resource
    Rapid micropropagation of Woodfordia fruticosa (L.) Kurz (Lythraceae), a rare medicinal plant (1994)
    Springer
    Plant cell reports 14 (1994), S. 55-58 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A rapid propagation method comprising initiation of in vitro shoot tip culture from field-grown flowering plants and reculture of the nodal segments of regenerated shoots in Schenk and Hildebrandt (1972) medium was developed for Woodfordia fruticosa (L.) Kurz., a rare medicinal shrub. A medium supplement of 6-benzylaminopurine (0.2 mg.l−1) induced high frequency (88%) development of axillary shoot buds (3.2) in 4–5 weeks. Subculture of the explants with multiple new shoots in fresh medium for 30 days yielded an even larger number (9.7) of shoots. Highest multiplication (26–35 shoots) was recorded when using culture initiation media with 0.5 mg.l−1 each of BAP and NAA followed by subculture in 0.2 mg.l−1 BAP. The shoot multiplication rate was further accelerated by reculturing 0.4–0.6 cm nodal segments of regenerated shoots in media with 1.0 mg.l−1 BAP. Shoot cuttings (3.5–7.0 cm) were rooted in 0.2 mg.l−1 IAA. Regenerated plants displayed uniform morphological, growth and flowering characteristics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233299
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  • 7
    Electronic Resource
    Electronic Resource
    Rapid propagation through shoot tip culture of Trichopus zeylanicus Gaertn., a rare ethnomedicinal plant (1995)
    Springer
    Plant cell reports 14 (1995), S. 708-711 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rapid micropropagation of Trichopus zeylanicus Gaertn. subsp. travancoricus Burkil ex Narayanan, a rare ethnomedicinal herb endemic to the Western Ghats of southern India, was achieved by culturing shoot tips (0.3–0.5 cm) of 2-month-old axenic seedlings on Woody Plant Medium. Among the cytokinins tested, only BAP induced callus-free multiple shoot bud formation, with a maximum of 8.5±0.4 buds per explant being obtained with 2.0 mg.l−1 BAP after 8 weeks of culture. Shoot tips containing proliferated buds were divided and subcultured on medium containing 0.2 mg.l−1 BAP to produce 12.0±1.0 shoots per explant in 6 weeks. Excision of buds after culture initiation, with subculture of the debudded basal tissue in 2 successive passages yielded 20.0±1.0 and 13.5±0.5 buds per explant respectively. Each bud cultured in turn for 4 weeks on WPM with 1.0 mg.l−1 BAP formed 3.8±0.4 secondary buds which were repeatedly recultured to increase bud production. Altogether this method enabled an estimated harvest of 7848 buds from a single shoot tip in 28 months. Shoots (3–5 cm) developed from bud cultures were rooted in half-strength WPM medium with 0.5 mg.l−1 each of NAA and IBA, and 90–100% of the rooted plants were established in the field after hardening. Micropropagated plants were grown to maturity free of defects in growth, morphological, flowering and seed set characteristics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232652
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  • 8
    Electronic Resource
    Electronic Resource
    Rapid clonal multiplication through in vitro axillary shoot proliferation of Aegle marmelos (L.) Corr., a medicinal tree (1998)
    Springer
    Plant cell reports 17 (1998), S. 422-426 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsAegle marmelos ; Clonal multiplication ; Organogenesis ; Rutaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rapid clonal multiplication of Aegle marmelos (L.) Corr. (Rutaceae), a medicinal tree, was achieved by enhanced axillary bud proliferation in young single-node segments of a 25-year-old tree cultured in Murashige and Skoog (MS) nutrient medium. Bud break was dependent on cytokinin supply, but the synergistic combination of 2.5 mg l–1 6-benzylaminopurine (BAP) and 1.0 mg l–1 indole-3-acetic acid (IAA) induced the formation of 12.1 shoots of up to 5.2 cm length in 48% of the explants after 7 weeks of culture. Explants of in-vitro-grown shoots – node, whole leaf, shoot tip and internode – were subcultured in the presence of 0.05–2.5 mg l–1 BAP to produce 11.3, 18.4, 5.3 and 3.2 shoots and shoot buds at a 100%, 70%, 95% and 40% rate respectively, in 7 weeks. Different shoot nodes and leaves were equally regenerative and adventitious organogenesis in the latter was confined to cut petiolar ends. Nodal explants responded most favourably at low BAP (0.05–0.1 mg l–1) and produced uniform (3.8–5.3 cm) shoots facilitating their simultaneous harvest for rooting. Repeated subculturing through five cycles of nodes and leaves of shoot cultures enabled continuous production of healthy callus-free shoots without any sign of decline. Shoot cuttings (3.0–5.2 cm) were best rooted in half-strength MS medium with 0.5 mg l–1 IAA (70%) or 10.0 mg l–1 indole-3-butyric acid (90%). Eighty-eight percent of the rooted plants were established in polybags after hardening.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050418
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  • 9
    Electronic Resource
    Electronic Resource
    Multiplication of the endangered Indian pitcher plant (Nepenthes khasiana) through enhanced axillary branching in vitro (1994)
    Springer
    Plant cell, tissue and organ culture 38 (1994), S. 69-71 
    Details
    ISSN: 1573-5044
    Keywords: ex situ conservation ; micropropagation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Axenic seedling-derived two- to three-node stem segments of Nepenthes khasiana Hook.f. were successfully cultured on Woody Plant Medium containing 2.2 μM benzyladenine to produce a 0.5–1.5 cm axillary shoot from each node in 7–8 weeks. The rapid growth along with the axillary branching of this shoot enabled amassing of 6–12 shoots during subculture. Excised shoots transferred to basal medium or rooted in medium containing 2.7 μM naphthaleneacetic acid produced typical pitchers at leaf tips. Rooted plants were established in pots at 90–95% survival rate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034447
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  • 10
    Electronic Resource
    Electronic Resource
    In vitro propagation of Rauwolfia micrantha, a rare medicinal plant (1996)
    Springer
    Plant cell, tissue and organ culture 44 (1996), S. 243-248 
    Details
    ISSN: 1573-5044
    Keywords: Medicinal plant ; micropropagation ; Rauwolfia micrantha ; rare plant conservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Shoot tip and single node explants from young shoots of 1-year old flowering plants of Rauwolfia micrantha Hook. f. were cultured on Murashige & Skoog (MS) medium variously supplemented with 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA). A combination of 13.2 μM BA and 2.68 μM NAA induced high frequency (77%) formation of up to 3 shoots from each node in 8 weeks. The regeneration of shoot tips from the field-grown plants and in vitro shoots placed horizontally differed. Repeated subculturing of the shoot tips and single nodes at 6-week intervals for over a year in combination of 4.4 μM BA and 0.27 μM NAA enabled mass multiplication of shoots without any evidence of decline. Rooting of the excised shoots on medium containing 2.6 μM NAA was preceded by callus formation. The rooted plants were removed off the callus, hardened off and 80% established in pots. Micropropagated plants displayed uniform morphological, growth, flowering, fruiting and seed germination characteristics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00048530
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