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  • 1
    Electronic Resource
    Electronic Resource
    Molecular technologies for biodiversity evaluation: Opportunities and challenges (1997)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 15 (1997), S. 625-628 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Better information on the degree and distribution of genetic variation is essential for developing more efficient ways of evaluating and conserving biodiversity. At present, an array of molecular techniques is available to detect diversity at the DNA level1, but the application of these ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0797-625
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  • 2
    Electronic Resource
    Electronic Resource
    PCR and sequencing from a single pollen grain (1996)
    Springer
    Plant molecular biology 31 (1996), S. 189-191 
    Details
    ISSN: 1573-5028
    Keywords: ITS ; PCR ; pollen grains ; rbcL ; sequencing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to eliminate the laborious step of DNA extraction preceding all studies within the field of plant molecular biology we attempted to do PCR amplifications directly on pollen grains. Successful PCR amplification was obtained in reactions including a single pollen grain from Hordeum vulgare or Secale strictum. Both the plastid gene encoding ribulose-1,5-biphosphate carboxylase/oxygenase (rbcL) and the nuclear-encoded internal transcribed spacer regions (ITS) and the 5.8S rDNA region were amplified and sequenced to verify PCR amplification.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020620
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  • 3
    Electronic Resource
    Electronic Resource
    A biometrical analysis of the South AmericanElymus glaucescens complex (Poaceae: Triticeae) (1989)
    Springer
    Plant systematics and evolution 166 (1989), S. 91-104 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Agropyron ; Elymus ; Biometrics ; Wells' distance diagram ; morphological variation ; Flora of S. America ; Patagonia ; Tierra del Fuego ; Falkland Islands
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Based on a simple multivariate analysis of the morphology of theAgropyron pubiflorum complex—A. patagonicum, A. antarcticum, A. fuegianum, andA. magellanicum—the variation pattern is shown to be clinal, and all taxa are included in a single, variable species. Currently the majority of species ofAgropyron is included inElymus and the correct name of this species isE. glaucescens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00937878
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  • 4
    Electronic Resource
    Electronic Resource
    Genome analysis, phylogeny, and classification (1989)
    Springer
    Plant systematics and evolution 166 (1989), S. 159-171 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Triticeae ; Biosystematics ; genome analysis ; chromosome homology ; phylogenetic reconstruction ; classification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The implications of genome analysis for evolutionary theory and systematics are treated. The precise relationship between the theoretical and operational definitions of chromosome homology is shown to be uncertain. It is pointed out that genera defined by genome analysis may be either monophyletic or non-monophyletic, and that the genus is not a basic unit of evolution. Characters obtained by genome analysis may be useful in a phylogenetic context, provided they are treated as all other characters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00935946
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  • 5
    Electronic Resource
    Electronic Resource
    Comparison of the Giemsa C-banded and N-banded karyotypes of twoElymus species,E. dentatus andE. glaucescens (Poaceae: Triticeae) (1994)
    Springer
    Plant systematics and evolution 192 (1994), S. 165-176 
    Details
    ISSN: 1615-6110
    Keywords: Poaceae ; Triticeae ; Elymus dentatus ; E. glaucescens ; Karyotype ; Giemsa C-banding ; N-banding ; genome differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The karyotypes ofElymus dentatus from Kashmir andE. glaucescens from Tierra del Fuego, both carrying genomesS andH, were investigated by C- and N-banding. Both taxa had 2n = 4x = 28. The karyotype ofE. dentatus was symmetrical with large chromosomes. It had 18 metacentric, four submetacentric and six satellited chromosomes. The karyotype ofE. glaucescens resembled that ofE. dentatus, but a satellited chromosome pair was replaced by a morphologically similar, non-satellited pair. The C-banding patterns of both species had from one to five conspicuous and a few inconspicuous bands per chromosome. N-banding differentiated the chromosomes of the constituent genomes by producing bands in theH genome only. TheS genomes of both species were similar with five metacentric and two satellited chromosomes having most conspicuous C-bands at telomeric and distal positions. They resembled theS genome of the genusPseudoroegneria. TheH genomes had four similar metacentric and two submetacentric chromosomes. The seventhH genome chromosome ofE. dentatus was satellited, that ofE. glaucescens nonsatellited, but otherwise morphologically similar. The C-bands were distributed at no preferential positions. TheH genome ofE. dentatus resembles theH genomes of some diploidHordeum taxa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00986250
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  • 6
    Electronic Resource
    Electronic Resource
    Simultaneous parsimony jackknife analysis of 2538rbcL DNA sequences reveals support for major clades of green plants, land plants, seed plants and flowering plants (1998)
    Springer
    Plant systematics and evolution 213 (1998), S. 259-287 
    Details
    ISSN: 1615-6110
    Keywords: Parsimony jackknifing ; phylogenetic analysis ; large data sets ; rbcL ; DNA sequences ; green plants ; land plants ; seed plants ; flowering plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ever-larger data matrices resulting from continuing improvements in DNA sequencing techniques require faster and more efficient methods of phylogenetic analysis. Here we explore a promising new method, parsimony jackknifing, by analyzing a matrix comprising 2538 sequences of the chloroplast generbcL. The sequences included cover a broad taxonomic range, from cyanobacteria to flowering plants. Several parsimony jackknife analyses were performed, both with and without branch-swapping and multiple random addition sequences: 1) including all positions; 2) including only first and second codon positions; 3) including only third positions; and 4) using only transversions. The best resolution was obtained using all positions. Removal of third positions or transitions led to massive loss of resolution, although using only transversions somewhat improved basal resolution. While branch-swapping improved both resolution and the support found for several groups, most of the groups could be recovered by faster simple analyses. Designed to eliminate groups poorly supported by the data, parsimony jackknifing recognizes 1400 groups on the basis of allrbcL positions. These include major taxa such as green plants, land plants, flowering plants, monocots and eudicots. We include appendices of supported angiosperm families, as well as larger groups.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00985205
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  • 7
    Electronic Resource
    Electronic Resource
    Genome analysis ofElymus angulatus andE. patagonicus (Poaceae) and their hybrids with N. and S. AmericanHordeum spp. (1991)
    Springer
    Plant systematics and evolution 174 (1991), S. 75-82 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Triticeae ; Elymus ; Hordeum ; Intergeneric hybrids ; genome analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The results of genome analysis of five hybrids, viz.Elymus patagonicus ×Hordeum procerum, E. patagonicus ×H. tetraploidum, E. angulatus ×H. jubatum, E. angulatus ×H. lechleri, andE. angulatus ×H. parodii, are reported. The genomic constitution ofHordeum tetraploidum andH. jubatum is best given as H1H1H2H2, ofH. lechleri andH. parodii as H1H1H2H2H4H4, ofH. procerum as H1H1H2H2H3H3, and ofElymus patagonicus andE. angulatus as SSH1H1H2H2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00937695
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  • 8
    Electronic Resource
    Electronic Resource
    The karyotype ofFestucopsis serpentini (Poaceae Triticeae) from Albania studied by banding techniques and in situ hybridization (1996)
    Springer
    Plant systematics and evolution 201 (1996), S. 75-82 
    Details
    ISSN: 1615-6110
    Keywords: Poaceae ; Triticeae ; Festucopsis ; Peridictyon ; Giemsa ; C-banding ; N-banding ; silver staining ; in situ hybridization ; FISH ; karyotype ; chromosome polymorphism ; nucleoli ; rDNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The karyotypes of two populations ofFestucopsis serpentini (2n = 2x = 14) endemic to Albania were investigated in detail by Giemsa C- and N-banding, AgNO3 staining, and in situ hybridization with an rDNA probe. The complements consisted of 14 large chromosomes, 10 metacentric and 4 SAT-chromosomes, a metacentric and a submetacentric pair. SAT-chromosomes from one population carried exclusively minute satellites, whereas SAT-chromosomes from another population also carried larger polymorphic satellites, suggesting a geographical differentiation. The existence of four chromosomes with nucleolus forming activity was established through AgNO3 staining; however, the rDNA probe additionally hybridized to intercalary positions in the short arms of two metacentric chromosomes revealing two inactive rDNA sites. C-banding patterns comprised from zero and up to four very small to larger, generally telomeric bands per chromosome giving low levels of constitutive heterochromatin. Similarities in chromosome morphology and C-banding patterns identified the homologous relationships of all chromosomes in one population, but of three pairs only in the other. Reliable identification of homologous chromosomes between plants was only possible for the SAT-chromosomes. A comparison between the C-banded karyotypes ofF. serpentini andPeridictyon sanctum supports their position in two genera.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00989052
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  • 9
    Electronic Resource
    Electronic Resource
    Molecular characterization and sequence polymorphism of the alcohol dehydrogenase 1 gene in Hordeum vulgare L. (1998)
    Springer
    Euphytica 102 (1998), S. 57-63 
    Details
    ISSN: 1573-5060
    Keywords: alcohol dehydrogenase 1 ; duplication ; Hordeum vulgare ; microsatellite ; partial DNA sequences ; sequence diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Partial sequences (appr. 900bp) spanning from exon 2 to 4 of the gene encoding alcohol dehydrogenase 1 were obtained from four varieties of cultivated barley, Hordeum vulgare ssp. vulgare, and from 21 accessions of its wild progenitor, H. vulgare ssp. spontaneum. Sequence diversity was low and restricted to the non-coding intron sequences. In the 648–660bp long intron 3 both a microsatellite locus and a major duplication (153/156bp) were found. Sequence diversity in H. vulgare ssp. spontaneum was slightly higher than in cultivated barley. In general the Adh1 gene displays only limited polymorphism and is not appropriate for seeking correlations between molecular and eco-geographical data.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018326320631
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  • 10
    Electronic Resource
    Electronic Resource
    ITS regions highly conserved in cultivated barleys (1996)
    Springer
    Euphytica 90 (1996), S. 233-234 
    Details
    ISSN: 1573-5060
    Keywords: internal transcribed spacers ; sequence diversity ; Hordeum vulgare ; barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Searching for DNA sequences variable enough to detect substitutions at the level of cultivars, the internal transcribed spacer regions, ITS1 and ITS2, of the nuclear ribosomal DNA were sequenced from ten cultivated varieties of barley, Hordeum vulgare L. However, all sequences were identical, and also identical to a previously published sequence from an unnamed variety.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023863
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