ISSN:
1076-5174
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Physics
Notes:
A method to measure the enrichment of deuterium in the hydrogens bound to C-6 of glucose has been refined and taken to the micro-scale. Glucose (≤0.2 mg) in small quantities of plasma samples is oxidized with sodium periodate. The formaldehyde formed contains the two hydrogens bound to C-6 of glucose. The formaldehyde is condensed with ammonia to form hexamethylenetetramine (HMT), which can be directly determined by gas chromatography/mass spectrometry. All of the 12 hydrogens of HMT are from the formaldehyde and therefore deuterium enrichment as low as 0.1% on hydrogens bound to C-6 of glucose can be measured with a coefficient of variation of less than 3%. The method can be used to quantify gluconeogenesis in vivo in humans by administering [2H2]O at an enrichment in total body water of only 0.5%, resulting in reproducibly measurable labeling of the hydrogens bound to C-6 of glucose. The method is presented because of its potential value for the measurement of gluconeogenesis in humans in vivo at safe and acceptable doses of [2H2]O.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jms.1190301111
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