ISSN:
1365-2958
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
,
Medicine
Notes:
Rhizobium meliloti DctD is believed to have three functional domains: an N-terminal, two-component receiver domain; and like other σ54-dependent activators, C-terminal and central domains for DNA binding and transcription activation. We have characterized a progressive series of M-terminal deletions of R meliloti DctD. The N-terminal domain was not needed for binding the dctA upstream activation sequence. Only 25% of the C-terminal end of the receiver domain was needed to significantly inhibit the central domain, and proteins lacking up to 60% of the N-terminal end of the receiver domain were‘inducible’in R. meliloti cells. We hypothesize that the W-terminal two-thirds of the DctD receiver domain augments and controls an adjacent subdomain for inhibiting the central domain.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2958.1994.tb00401.x
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