ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
The structure of methionyl-tRNA_f^{\rm Met} formyltransferase from E. coli, a monomeric protein of 34 kDa, has previously been determined at 2.0 Å resolution. In the present work, this enzyme was crystallized as a complex with its macromolecular product, the initiator formyl-methionyl-tRNA_f^{\rm Met} (25 kDa). Polyethylene glycol 5000 monomethylether was used as a precipitating agent. The crystals are orthorhombic and have unit-cell parameters a = 201.7, b = 68.1, c = 86.4 Å. They belong to space group P21212 and diffract to 2.8 Å resolution. The structure is being solved with the help of a mercury derivative.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444998011780
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