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  • 1
    Publication Date: 2020-02-12
    Type: info:eu-repo/semantics/article
    Format: application/pdf
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  • 2
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effects of compost addition and simulated solarisation of soil on the survival of Ralstonia solanacearum biovar 2 strain 1609, as well as on the structure of indigenous soil bacterial communities, were analysed. In addition, effects on the invasion of susceptible test plants by strain 1609 were assessed. In untreated soil in microcosms and the field, strain 1609 showed slow progressive declines, from 106–107 to roughly 104–105 CFU per g dry soil in around 60 days. When these soils were used in suppressiveness tests, a majority of plants developed symptoms of wilting and revealed the presence of the pathogen in their lower stem parts, as evidenced by immunofluorescence colony staining (IFC) and polymerase chain reaction (PCR). Solarisation of unamended soil did not drastically affect R. solanacearum survival or plant invasiveness. However, the addition of household compost resulted in enhanced R. solanacearum population decline rates, as well as reduced numbers of diseased plants in suppressiveness tests. Combined solarisation and compost addition yielded differential results between microcosms and the field. Some healthy-looking plants, primarily from soils treated with compost, revealed the latent presence of strain 1609 in the lower stem parts. The eubacterial and β-subgroup proteobacterial communities in the differentially treated soil microcosms were rather stable, as evidenced by analysis of PCR-denaturing gradient gel electrophoresis (DGGE) generated molecular profiles. However, compost amendment clearly induced changes in these communities, which were detectable until the end of the experiment; two major bands, affiliated with Variovorax paradoxus and Aquaspirillum psychrophylum, were associated with the compost amendment. The decrease in abundance of R. solanacearum in the compost-amended soils was confirmed by the DGGE profiles.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 11 (1955), S. 24-25 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Résumé De nombreuses souches d'actinophages peuvent être mises en évidence et isolées, en ensemençant une série deStreptomyces spp. sur des milieux gélosés à base d'extraits aqueux de sol ou de purin préalablement stérilisés par filtration.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 50 (1994), S. 442-446 
    ISSN: 1420-9071
    Keywords: Glucagon ; endocrine pancreas ; exocrine pancreas ; islet-acinar axis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Intravenous glucagon inhibits exocrine pancreatic secretion in vivo, but exogenous glucagon does not affect exocrine secretion in vitro. Recent work, however, suggested that endogenous glucagon may be involved in the regulation of exocrine secretion even in vitro. We therefore investigated the effects of exogenous and endogenous glucagon on exocrine secretion by the isolated perfused rat pancreas in the presence of 1.8 mM glucose. Exogenous glucagon did not affect CCK-stimulated amylase output. 20 mM arginine stimulated glucagon release, but did not affect basal enzyme secretion. CCK-stimulated amylase output, however, was significantly inhibited in the presence of arginine. This inhibitory effect of arginine on exocrine pancreatic secretion could be blocked by glucagon antibodies, but not by nonspecific gammaglobulins. Thus exogenous glucagon failed to affect exocrine pancreatic secretion in vitro, but endogenously released glucagon or a glucagon-like peptide inhibited amylase release in the isolated perfused pancreas. We conclude that glucagon or a glucagon-like peptide may be a mediator in the islet-acinar axis.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 51 (1995), S. 556-560 
    ISSN: 1420-9071
    Keywords: Camostate ; endocrine and exocrine pancreas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract It is well known that oral administration of camostate induces hyperplasia and hypertrophy of the rat pancreas. It is not clear, however, whether pancreatic hormone and enzyme secretion are affected by camostate treatment. In rats, daily administration of 200 mg camostate/kg b. wt for 14 days significantly increased pancreatic weight and pancreatic content of DNA, protein, amylase, lipase, trypsin and chymotrypsin, as well as the amount of insulin, glucagon and somatostatin. In the intact animal, blood glucose levels and serum concentrations of insulin and glucagon in response to an oral glucose load were not impaired after camostate treatment. In the isolated perfused pancreas, however, insulin and glucagon secretions were reduced, whereas somatostatin release was not affected. The volume of pancreatic juice produced by the unstimulated isolated perfused organ, as well as protein and enzyme secretion, were increased after camostate treatment. Likewise, the isolated perfused pancreas from camostate-treated rats secreted a larger volume of pancreatic juice and more protein in response to cholecystokinin (CCK), while enzyme secretion was affected in a non-parallel manner: amylase release was markedly reduced, lipase release was unchanged, and release of trypsin and chymotrypsin was increased.
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  • 6
    ISSN: 1420-9071
    Keywords: Key words. Silibinin; cyclosporin A; endocrine pancreas; exocrine pancreas; insulin; amylase.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Silymarin can be extracted from the milk thistle, and silibinin is the main component of the plant extract. Possibly due to their antioxidant and membrane-stabilizing properties, the compounds have been shown to protect different organs and cells against a number of insults. Thus liver, kidney, erythrocytes and platelets have been protected from the toxic effects of ethanol, carbon tetrachloride, cold ischemia and drugs, respectively. The effect of silibinin on endocrine and exocrine pancreas, however, has not been studied. We therefore investigated whether silibinin treatment attenuates cyclosporin A (CiA) toxicity on rat endocrine and exocrine pancreas. Groups of 15 male Wistar rats were treated for 8 days with CiA and/or silibinin. On day 9, endocrine and exocrine pancreatic functions were tested in vitro. At the end of the treatment period, blood glucose levels in vivo were significantly higher in rats treated with CiA, while silibinin did not affect glucose levels. In vitro, insulin secretion was inhibited after treatment with silibinin, but amylase secretion was not affected. After treatment with CiA both insulin and amylase secretion were reduced. Silibinin and CiA had an additive inhibitory effect on insulin secretion, but silibinin attenuated CiA-induced inhibition of amylase secretion. Despite CiA treatment, amylase secretion was in fact restored to normal with the highest dose of silibinin. Thus silibinin inhibits glucose-stimulated insulin release in vitro, while not affecting blood glucose concentration in vivo. This combination of effects could be useful in the treatment of non-insulin-dependent diabetes mellitus. Furthermore, silibinin protects the exocrine pancreas from CiA toxicity. As this inhibitory effect is probably unspecific, silibinin may also protect the exocrine pancreas against other insult principles, such as alcohol.
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  • 7
    ISSN: 1572-9699
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract No major differences have been found in series of Staphylococcus aureus strains which reverted from ‘L’-phase, either by pyrolysis mass spectrometry or by phage-typing or sensitivity testing. In ‘L’-phase they have been subcultured for a long time or transformed/reverted many times into/from ‘L’-phase. Plasmids were lost during transformations/reversions, but there was some difference between the tetracycline-connected plasmids on the one hand and the erythromycin-connected ones on the other.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Flow, turbulence and combustion 3 (1954), S. 417-450 
    ISSN: 1573-1987
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Summary A clearer conception of the analogies is pursued by treatment of hydraulic systems as distinct from mechanical systems, interpretation of acoustic as mixed mechanic-hydraulic systems, analysis of the operation of piston mechanisms, and consideration of gyro-elements.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Flow, turbulence and combustion 1 (1949), S. 169-197 
    ISSN: 1573-1987
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Summary When investigating the influence of an acceleration upon the velocity distribution and upon the resistance in a tube or canal, a distinction can be made between slowly varying motions where the resistance dominates, and quickly varying motions where the inertia dominates. When the motion varies quickly, practically all the liquid moves bodily, and the resistance only affects a small region near the walls. When the motion varies slowly, the velocity distribution differs from that of steady flow in that there is a phase lag of the central layers with respect to the peripheral layers. The following special types of motion were studied for laminar flow: forced oscillations in a round tube; forced oscillations in a crevice; the starting motion by a constant drop of potential in a round tube; free oscillations in a round tube. For free oscillations in a U-tube, the theory is checked by comparison with experiments (table I). General slow motion was studied in the following cases: laminar flow in tubes of elliptical (circular) and rectangular (square) cross-section, or in open canals of rectangular cross-section; fully developed turbulent flow in round tubes and wide open canals. The relation between drop of potential and total flow can be represented by the impedance of the tube or canal. For quick laminar motions the impedance is given by (52) and (53) and characterized by the high frequency inertanceH. For slow laminar motions the impedance is given by (49) and (50) and characterized by the resistanceR and the low frequency inertanceL. For slow turbulent motions the relation between drop of potential and total flow is given by (73). The low frequency inertance is always greater than the high frequency inertance. The difference represents the change in the resistance caused by the influence of the acceleration upon the distribution of the velocity. This difference betweenL andH amounts to 33% for laminar flow in a round tube, 38% in a square tube, and 20% in a crevice or a wide open canal. For turbulent flow it ranges from 1 to 8% in a rough round tube, from 1/2 to 3% in a smooth round tube, from 1/2 to 3 1/2% in a rough open canal or crevice, and from 1/4 to 1 1/2% in a smooth open canal or crevice (table III). For laminar flow the ratioL: H is equal to the ratio of the mean square and the square mean velocity in a steady flow.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Antonie van Leeuwenhoek 29 (1963), S. 211-211 
    ISSN: 1572-9699
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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