ISSN:
0884-3996
Keywords:
adenyl compounds
;
DNA
;
chemiluminescence
;
glyoxal derivatives
;
heteropoly acid
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
A chemiluminescence (CL) determination of adenyl compounds is described. CL derivatization of adenyl compounds with methylglyoxal dimethyl acetal was performed in the presence of tungstosilicic acid and propan-2-ol. CL from adenyl compounds was produced by hydrogen peroxide and L-cysteine ethyl ester in DMF and water. The proposed method is highly sensitive and specific to compounds containing adenine. Adenine was determined in the range 1.0 × 10-3 -5.0 × 10-8 M with the detection limit of 3.0 × 10-8 M (150 fmol per assay). The method was applied to the determination of DNA and detection limits of a few nanograms of DNA achieved. © 1998 John Wiley & Sons, Ltd.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
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