ISSN:
1573-0603
Keywords:
Airway circulation
;
Bronchial artery
;
Cell culture
;
Endothelial
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Methods are described for the isolation of endothelial cells from sheep bronchial artery by treatment with collagenase. Cells obtained from the perfusate of the collagenase-treated vessel are cultured. Identified by their cobblestone morphology, endothelial cell colonies of approximately 50 cells are selected by a cloning cylinder and subcultured using trypsin. Endothelial cells are characterized by the formation of vessel-like tubes on basement membrane and by incorporation of DiI-acetylated-low density lipoprotein.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00981883
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