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  • 1
    Electronic Resource
    Electronic Resource
    EFFECT OF POSTMORTEM AGING ON CHICKEN MUSCLE FIBRILS (1973)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 38 (1973), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1973.tb02129.x
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  • 2
    Electronic Resource
    Electronic Resource
    EFFECT OF POSTMORTEM AGING ON CHICKEN BREAST MUSCLE SARCOPLASMIC RETICULUM (1973)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 38 (1973), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: FSR (fragmented sarcoplasmic reticulum) isolated from chicken breast muscle (Pectoralis major) at 0 hr, 48 hr and 7 day postmortem was purified using linear density gradient centrifugation. The Ca++accumulating ability of the FSR was found to increase with postmortem aging. No loss in ATPase activity was noted nor was any significant change observed in the SDS-gel electrophoresis patterns of the proteins with postmortem aging. The FSR from the aged muscle contained a higher proportion of phospholipids These studies indicate that the Ca++ sequestering properties of sarcoplasmic reticulum from chicken breast muscle are not impaired during postmortem aging.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1973.tb02848.x
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of nerve stimulation, denervation, and duct ligation, on kallikrein content and duct cell granules of the cat's submandibular gland (1977)
    Springer
    Cellular and molecular life sciences 33 (1977), S. 746-748 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Various procedures which reduce or deplete the kallikrein content of the cat's submandibular gland correspondingly reduce the number of apical granules in the striated duct cells. The kallikrein content is greatly reduced after chronic parasympathetic but not after sympathetic nerve section which suggests that the parasympathetic innervation is required for synthesis or storage of this enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01944167
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  • 4
    Electronic Resource
    Electronic Resource
    On the mechanism of electrical coupling between cells of earlyXenopus embryos (1976)
    Springer
    The journal of membrane biology 27 (1976), S. 393-408 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The mechanism of electrical coupling between cells of earlyXenopus embryos has been studied by examination of the nonjunctional membrane resistances and capacitances as a function of cleavage stage, the junctional and nonjunctional membrane resistances as functions of time during the first cleavage, and the electrical properties of the primitive blastocoel. The changes in membrane resitances and capacitances during the first two cleavages may be completely explained by the addition of new membrane, identical in specific resistance and capacitance to the original membrane, at a constant rate to furrows which are electrically connected to the perivitelline space. Microelectrode recording from the primitive blastocoel indicates that there is no electrical difference detectable between it and the perivitelline space. These results are discussed in the context of current theories of the mechanism of intercellular electrotonic coupling.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01869148
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  • 5
    Electronic Resource
    Electronic Resource
    Aspects of furrow membrane formation in the cleaving Drosophila embryo (1975)
    Springer
    Cell & tissue research 156 (1975), S. 463-474 
    Details
    ISSN: 1432-0878
    Keywords: Embryonic cleavage ; Drosophila embryo ; Membrane growth ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary During blastoderm formation, the Drosophila embryo produces a large area of new membrane to accommodate the simultaneous demands of approximately four thousand newly cleaved cells. The embryo was examined with the electron microscope at various stages during cleavage in order to investigate the high membrane forming capacity of these cells. Embryos were subjected to the histochemical procedure for the demonstration of thiamine pyrophosphatase. The enzyme was present in the cisternae of the endoplasmic reticulum, Golgi bodies and the nuclear envelope. No activity could be demonstrated on the furrow surface or at the furrow tip despite closely adjacent reactive cisternae. It is concluded that the endoplasmic reticulum and Golgi bodies are not major contributors to the new surface. Lamellar bodies were frequently observed in the cytoplasm of all stages examined. The bodies showed a lamellar periodicity of approximately 3.5 nm, and were ultrastructurally similar in appearance after a variety of fixation procedures. The distribution of these bodies was markedly related to the stage of blastoderm formation. Before the commencement of cleavage, lamellar bodies were very prominent within a region 4–5 μm. below the cell surface. As cleavage progressed, the bodies became sparse or absent from this region but were apparent at the base of the blastoderm cells or in the sub-blastoderm region, where they were not previously present. Lamellar bodies with leaflets closely associated with, or in apparent continuity with, the cleavage furrow membrane were frequently observed. In these regions the lamellar periodicity was the same as the thickness of the membrane laminae. It is suggested that these bodies play a role in the synthesis of new membrane in the furrow. Intercellular contact specializations between the developing membranes of the furrow were restricted to incipient desmosones and point contacts where the intercellular gap was reduced to 3 nm or less.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225106
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  • 6
    Electronic Resource
    Electronic Resource
    Cytomembranes in first cleavage xenopus embryos (1974)
    Springer
    Cell & tissue research 154 (1974), S. 189-209 
    Details
    ISSN: 1432-0878
    Keywords: Membrane relationships ; Xenopus embryos ; Golgi body ; Endoplasmic reticulum ; Lipid droplets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure and interrelationships of the Golgi body, endoplasmic reticulum and lipid droplets have been studied in the first cleavage Xenopus embryos. Lipid droplets, usually spherical or sometimes multilobed, did not have a discernible limiting membrane, although some had an incomplete electron dense partition. The Golgi bodies and endoplasmic reticulum were seen continuous with lipid droplets and the profiles indicated a probable formation of these membranes from lipid droplet material. Rough endoplasmic reticulum (ER) mainly consisted of paired tubular cisternae and vesicles containing filamentous material that gave a fringed appearance. The relationships of paired cisternae with the Golgi body suggested a transformation of ER membranes into the Golgi body membranes. In addition, paired ER cisternae showed a close apposition with the limiting membrane of the yolk platelet. Lone ER cisternae that contained moderately electron dense material instead of filaments were also present and showed numerous associated vesicles near the Golgi body. The Golgi body showed several morphological forms including a single fenestrated cisterna, two to four flat or cup-shaped cisternae, or up to seven cisternae, some of which were dilated and similar to fringed ER in appearance. These forms could be different developmental stages of the organelle. Coated vesicles were seen continuous with the cisternae of the Golgi body. A probable route for the assembly of the cell surface material has been proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223164
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  • 7
    Electronic Resource
    Electronic Resource
    Development of the basal lamina and extracellular materials in the early chick embryo (1979)
    Springer
    Cell & tissue research 198 (1979), S. 527-537 
    Details
    ISSN: 1432-0878
    Keywords: Chick embryo ; Cell surface ; Basal lamina ; Extracellular materials ; Morphogenesis ; Tannic acid ; Glutaraldehyde fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Chick embryos at developmental stages up to primitive streak formation were fixed in a mixture of tannic acid and glutaraldehyde. A basal lamina was present in the unincubated embryo and consisted of a lucent lamina interna and a lamina densa. At the primitive streak stage the lamina densa showed a periodicity of stained elements. Densely stained materials were present on the cell surfaces lining the cavity between the epiblast and endoblast, and on the mesoderm cells within this cavity. Considerable amounts of extracellular material were observed in the cavity. Hyaluronidase treatment removed the cell surface and extracellular material, indicating that hyaluronic acid is a major component. This enzyme disrupted the basal lamina, leaving a fibrillar remnant with no periodic structure. It is therefore suggested that the dense periodicities consist of glycosaminoglycan built on an enzyme-resistant framework which is probably collagen. Enzyme-resistant fibrils, presumably collagen precursors, are present elsewhere within the tissue spaces.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234196
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  • 8
    Electronic Resource
    Electronic Resource
    Ultrastructural cytochemical localization of adenylate cyclase in the early chick embryo (1987)
    Springer
    Cell & tissue research 247 (1987), S. 465-468 
    Details
    ISSN: 1432-0878
    Keywords: Adenylate cyclase ; Cytochemistry ; Gastrulation ; Ultrastructure ; Chick embryo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Adenylate cyclase activity was localized in various tissues of the early chick embryo using an ultrastructural histochemical technique. Reaction product was deposited on the lateral plasma membrane of all cells, but with a preferential localization at the apical terminal complex in the epiblast. There was no activity associated with the free surfaces of these or other cells in the embryo. Intracellular deposits were found in all cells associated with the endoplasmic reticulum, nuclear envelope and Golgi bodies. In the last organelle, the deposit was sometimes observed to be distributed through the stack in a non-uniform way, with the heaviest deposits occurring at the forming face. No clear difference could be detected between the cytochemical activity associated with cells in various regions of the embryo, or with embryos at different stages of early development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218330
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  • 9
    Electronic Resource
    Electronic Resource
    The mechanism of sensory transduction in the sensilla of the trochanteral hair plate of the cockroach, Periplaneta americana (1979)
    Springer
    Cell & tissue research 198 (1979), S. 159-174 
    Details
    ISSN: 1432-0878
    Keywords: Neurobiology ; Neuroanatomy ; Mechanoreceptors ; Hair plate ; Sense organs ; Sensory transduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The trochanteral hair plate of the cockroach leg contains approximately 60 hair sensilla that are deflected by a joint membrane during flexion of the leg. Previous work has shown that the organ is a mechanoreceptor which limits leg flexion during walking by reflex connections to flexor and extensor motoneurons. Functional analysis of the largest sensilla has shown that their behaviour may be well approximated by a velocity detector followed by a unidirectional rectifier. We report here the results of an examination of the largest sensilla by scanning and transmission electron microscopy in an attempt to correlate the structure with the known functional elements. Each hair is innervated by a single sensory dendrite which is surrounded by an electron dense dendritic sheath. The dendrite terminates below the hair shaft in a tubular body containing a parallel array of microtubules embedded in an electron dense matrix, while the dendritic sheath extends beyond the tubular body to form the walls of the ecdysial canal. At the proximal end of the tubular body the dendritic sheath and sensory dendrite are anchored to the cuticular socket by a fibrous dome which seems to form a fulcrum around which the tubular body can be deflected by movements of the hair. We suggest that the basis for the detection of velocity may be mechanical differentiation by a fluid space between the dendritic sheath and the tubular body. The structure is also discussed with relation to the mechanism of sensory transduction and the possible causes of the unidirectional sensitivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234843
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  • 10
    Electronic Resource
    Electronic Resource
    Morphological differentiation of an embryonic epithelium in culture (1981)
    Springer
    Cell & tissue research 220 (1981), S. 539-548 
    Details
    ISSN: 1432-0878
    Keywords: Cultured epithelium ; Epiblast ; Chick embryo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This communication reports the results of a morphological study of three-day old cultures of epiblast tissue from the early chick embryo. The most striking feature of these cultures was the appearance of “domes” or elevated blister-like structures, composed of a single layer of cells which were morphologically distinct from the remaining cells in the culture. The domes arose in high-density areas of the culture. Their roofs were lined by basal laminae that did not develop in other areas of the culture. In several morphological respects, the cells of the dome roof closely resembled the epiblast in vivo. This was in contrast to the cells spread on the substratum in sparse regions of the culture, which did not. Each dome was surrounded by a dense ring of multilayered ruffling cells which appeared to give rise to both the dome roof and to fibroblast-like cells that spread on the substratum beneath the dome. Fibroblast-like cells also developed in discrete patches in other regions of the culture. In other tissues, dome formation has been attributed to fluid transport by the epithelium; in the present case it is also possible to invoke the capacity of the epiblast to fold, as contributing to the mechanism of dome formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216757
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