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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Comparative Biochemistry and Physiology -- Part A: Physiology 103 (1992), S. 817-822 
    ISSN: 0300-9629
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Gene 120 (1992), S. 183-190 
    ISSN: 0378-1119
    Keywords: DNA and protein homologies ; Recombinant DNA ; gene amplification ; interspersed repeats ; molecular cloning ; nucleotide sequence ; reverse transcription
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0965-1748
    Keywords: Culex pipiens ; Gene amplification ; Insect transposons ; LINE ; Molecular biology ; Mosquitoes ; Repetitive sequences ; Reverse transcription
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-5225
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Summary Kraft delignification kinetics has been modelled on the basis of a first order decay process with a time-dependent rate constant. A generalized severity parameter derived from this kinetic model, Roh, has been applied to describe the lignin solubilization during alkaline (soda and Kraft) and bisulphite pulping of different wood species. The model has been succesfuly applied to data sets available from the literature. Our approach has combined the main process variables (temperature, time and chemical load) into a single parameter, Roh, which is then used as a reaction ordinate to map the changes in chemical composition and physical properties. An extension of the initial formulation of the Roh parameter has been made to cover the situations where the catalytic system is composed by two active chemical species, as in the Kraft process.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Plasmid 22 (1989), S. 151-159 
    ISSN: 0147-619X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 78 (1989), S. 505-512 
    ISSN: 1432-2242
    Keywords: Agrocybe aegerita ; Edible mushroom ; Protein extraction ; Protein mapping ; Genome expression variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A procedure suitable for the extraction and mapping of total proteins from the basidiomycete, Agrocybe aegerita, was developed. A. aegerita mycelia were fragmented either with a Dangoumeau grinder, an X-press bomb or a sonicator and the efficiency of these three disruption methods were compared. The extraction buffer composition was optimized to avoid proteolytic activities. 2D-SDS-PAGE analysis of protein extracts showed that the rate of reproducibility depending on extractions and electrophoretic separations was always greater than 96% for all strains. The differences in efficiency observed between the breaking procedures indicate that the A. aegerita cell wall is more mechanically resistant than that of other basidiomycetes. The efficient action of protease inhibitors (PMSF and SDS) showed that A. aegerita mycelia contains numerous and/or highly active proteases. Reproducibility of protein extraction and separation methods allowed the establishment and the comparison of standard maps. Qualitative and quantitative variations in gene products between a wild dikaryotic strain and 11 homokaryotic strains from its progeny were examined. The genetic diversity, determined by comparing the distribution of proteic variations in 11 homokaryons from the same progeny, was comparable to that observed between co-isogenic homokaryons of another basidiomycete.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-2932
    Keywords: bean ; chemometrics ; hydroxycinnamic acid ; leaf polyphenolics ; open-top chamber ; ozone ; Phaseolus vulgaris ; phenolic fingerprint
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract From sowing till emergence of the fourth trifoliolate leaf, beans were exposed for seven hr a day to four ozone concentrations in open-top chambers: filtered air (FA), non-filtered air (NF), non-filtered air plus 30 ppb ozone (NF+) and non-filtered air plus 60 ppb ozone (NF++). Controls in ambient air of open plots (AA) were also available. The free polyphenolic pool of the first trifoliolate leaf was systematically investigated using HPLC. The groups of phenolics to which the three main chromatographic signals belonged were identified as hydroxycinnamic acid derivative for peak No 1 and flavonoids for peaks Nos 4 and 6. The metabolic regulation of phenolic accumulation could be recognized as different clusters based on specific phenolic patterns that reflected some of the tested atmospheric conditions. The comparison of AA and NF conditions revealed a ‘chamber effect’. FA and NF atmospheres gave rise to almost the same phenolic equilibriums dominated by a hydroxycinnamic acid derivative (about 53% of the total phenolics). Ozone addition was associated with a regular reduction of the percentage of peak No 1: about 40% for NF+ and 19% for NF++. Therefore, the significant decrease of the vacuolar storage of this phenolic compound (from 23 mg g-1 dry weight for NF to about 3 mg g-1 dry weight for NF++) can be regarded as a useful tool for monitoring long-term exposure to elevated ozone concentrations.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Genetica 93 (1994), S. 139-148 
    ISSN: 1573-6857
    Keywords: adaptation ; Aedes aegypti ; biodiversity ; Culex pipiens ; gene amplification ; insect ; LINE ; mobile elements ; molecular evolution ; mosquitoes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Most of the transposons so far characterized from mosquito genomes are retroelements which seem to be distributed worldwide. The Juan transposons constitute a family of non-LTR retroelements, or LINE-retroposons, which are dispersed in the genomes of several mosquito species. Three different Juan subfamilies have been characterized, each being amplified in the genomes of many strains, if not all, of a given mosquito species. These subfamilies have been designated respectively Juan-C inCulex pipiens, Juan-Ct inCulex tarsalis and Juan-A inAedes aegypti. A large number of the Juan retroposons which are amplified in the mosquito genomes are apparently full-length copies and potentially encode the enzymes necessary for their transposition, a nucleic acid binding protein and a reverse transcriptase. However, these complete Juan copies seem to be most frequently transcriptionally silent in insects reared under laboratory conditions. A few of them are transcribed inC. pipiens cells grownin vitro, but from an external promoter, the Juan-C specific RNA being fused to an upstream RNA sequence. Therefore, the transcription of Juan retroposons seems to depend on external promoters which are most frequently inactive. The occurrence and distribution of Juan retroposon subfamilies among mosquito species do not reflect the phylogeny of these species. Furthermore, complete Juan-C and Juan-A copies which are reiterated in strains collected from regions covering different continents are nearly identical. Juan-C copies belonging to geographically differentC. pipiens strains display low levels of divergence between their nucleotide sequences and many of the mutations which have occurred among these copies do not alter their coding potential. These results indicate that the Juan retroposons occur as homogeneous subfamilies distributed worldwide and that selective constraints against amino acid change have been acting recently on these elements, despite the fact that they are now highly repeated through mosquito genomes. Therefore, Juan transposons have most probably been recently amplified in mosquito genomes. Each subfamily may have been amplified from one master element present in a unique population which has since spread worldwide. Alternatively, this amplification may have arisen in many mosquito populations, but from highly conserved master elements submitted to selection pressures. Horizontal transfers between species may also have contributed to the spread of these transposons.
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  • 9
    Publication Date: 2002-08-01
    Print ISSN: 0018-3768
    Electronic ISSN: 1436-736X
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Published by Springer
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  • 10
    Publication Date: 2017-06-01
    Print ISSN: 1126-6708
    Electronic ISSN: 1029-8479
    Topics: Physics
    Published by Springer
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