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  • 1
    Electronic Resource
    Electronic Resource
    Increased capsaicin content in PFP-resistant cells of chili pepper (Capsicum annuum L.) (1990)
    Springer
    Plant cell reports 8 (1990), S. 617-620 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cell suspensions of chili pepper (Capsicum annuum L.) were subjected to a selection process on semisolid medium containing the amino acid analog p-fluorophenylalanine (PFP). Four cell lines with different degrees of resistance were selected and suspension cultures were established from each of them. Resistance was retained even after 75 days of culture in the absence of PFP. PFP-resistant cell lines accumu lated higher levels of capsaicin than sensitive lines even after prolonged culture in PFP-free medium. Capsaicin production in non-selected cells was only 26.8% of that found in one cell line resistant to 500 μM PFP. The capsaicin content in the non-selected cell suspension and in one of the resis tant cell lines was 6.7% and 24.9% respectively, that of fruits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270067
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  • 2
    Electronic Resource
    Electronic Resource
    NaCl-resistant variant cells isolated from sweet potato cell suspensions (1985)
    Springer
    Plant cell, tissue and organ culture 5 (1985), S. 3-12 
    Details
    ISSN: 1573-5044
    Keywords: sweet potato ; Ipomoea batatas ; cell suspensions ; salt-resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Salt-resistant cells of sweet potato (Ipomoea batatas L.) were selected by subculturing cell suspensions (11 transfers at 15-day intervals) in MS medium supplemented with 1% NaCl (170.9 mM NaCl). Selected cells showed a brownish pigmentation, and exhibited morphological changes (they were smaller and rounder than non-selected cells). The change in coloration was reversible when the selected cells were subcultured in medium without NaCl. The reduction in size was partially reversed but the change in form was not reversible when selected cells were subcultured 5 times at 15-day intervals in the absence of NaCl. Selected cells exhibited NaCl-tolerance when they were cultured in medium with 1% NaCl and subsequently transferred to NaCl free medium for 3 passages. This finding suggests that the acquired trait is stable for at least 3 passages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033564
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  • 3
    Electronic Resource
    Electronic Resource
    Acmella oppositifolia micropropagation by single-node culture (1996)
    Springer
    Plant cell, tissue and organ culture 45 (1996), S. 281-282 
    Details
    ISSN: 1573-5044
    Keywords: Acmella oppositifolia ; Compositae ; medicinal plant ; micropropagation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Acmella oppositifolia plantlet formation was achieved by subculturing single-node explants on Murashige and Skoog medium without growth regulators. The explants from 1-month-old in vitro plantlets produced shoots over a 7-day culture period. From these in vitro cultured nodes readily rooted shoots elongated on auxin-free MS medium. Plants produced were easily acclimatized and subsequently flowered in a greenhouse. This species is of medicinal value in tropical America from Mexico to Colombia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043644
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