ISSN:
1432-072X
Keywords:
Key words: Fatty acids –Mortierella alpina–ω3-Desaturation – Mutation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract. A mutant considered to be defective in the conversion of n-6 to n-3 fatty acids (ω3-desaturation) was derived from a Δ5-desaturation-defective mutant (Mut44) of Mortierella alpina 1S-4, after treating its spores with N-methyl-N′-nitro-N-nitrosoguanidine. This mutant cannot produce 8(Z),11(Z),14(Z),17(Z)-eicosatetraenoic acid or any other n-3 fatty acids, of which about 10% was found in its parental strain upon cultivation at 12 °C. The mutant's growth rate was comparable to that of the parental strain when grown at 28 °C, but it became much slower when the mutant grew at 12 °C, at which the lag phase for Mut44 was about 2 d but 5 d for the mutant.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00303586
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