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  • 1
    Electronic Resource
    Electronic Resource
    Temperature conditional cAMP-requiring mutant strains ofChlamydomonas reinhardtii arrest in G1 and are rescued by added cAMP (1996)
    Springer
    Protoplasma 192 (1996), S. 159-167 
    Details
    ISSN: 1615-6102
    Keywords: Adenylate cyclase ; Cell cycle ; Chlamydomonas reinhardtii ; Cyclic AMP ; Cyclic nucleotide phosphodiesterase ; Mutant strains
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three independent isolates ofChlamydomonas, selected for caffeine resistance, were found to arrest in G1 phase, as determined by quantitative fluorescence measurements of DNA, when grown at a non-permissive temperature. This cell cycle arrest correlated with lowered levels of cAMP and of adenylate cyclase activity. The arrested cells could be rescued by added cAMP but not AMP, hence the defect was not one of general purine metabolism. Back-crosses to wild type revealed that the phenotypes observed result from a combination of three separable mutations. It is clear that the mutations define functions that are more stringently required for cell division than for growth since the mutant strains are able to grow up to fifteen times normal size while blocked at the non-permissive temperature. The possible interaction of cAMP dependent events with division is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01273888
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation and partial characterization of conditional cell division cycle mutants inChlamydomonas (1995)
    Springer
    Protoplasma 186 (1995), S. 149-162 
    Details
    ISSN: 1615-6102
    Keywords: Algae ; Cytoskeleton ; Microtubules ; Microtubule organizing centres ; Mutation ; Temperature-sensitive
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have isolated a number of temperature conditional cell division cycle mutants of the unicellular plantChlamydomonas reinhardtii that are defective in single nuclear genes. Cells grow and divide normally at the permissive temperature (21 °C), but arrest in division at the restrictive temperature (33 °C). We have characterized these mutants using DNA probes and immunofluorescence techniques to localize cytoskeletal and microtubule organizing centre proteins. We describe here 3 broad classes of cell cycle mutation which result in cell cycle arrest with: unreplicated DNA (G1 arrest), duplicated DNA (G2 arrest) and multiple nuclei due to defective cytokinesis (cytokinesis arrest). The continuation of nuclear division in mutants blocked in cytokinesis provides support of an earlier hypothesis that stage specific events in theChlamydomonas cell cycle are arranged in separate dependent sequences. The mutants isolated in the present study provide insights into the role of cytoskeletal proteins in the coordination of plant cell division and the means to investigate the molecular mechanisms whereby division by multiple fission is controlled in the unicellular plantChlamydomonas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01281325
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