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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 46 (1981), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Sixty-three samples of variety meats were examined for the presence of Yersinia enterocolitica. Surface swab samples in a modified selenite enrichment broth were incubated at 23°C for 2 and 3 days and then streaked onto MacConkey agar with Tween 80 or refrigerated bismuth sulfite agar, with and without a potassium hydroxide preplating treatment. The organism was isolated from 2 of 23 porcine throat swabs but was not isolated from 40 bovine tongue, kidney, heart, or liver swabs. The isolates from the throat swabs were classified into Nilehn and Wauters biotypes 3 or 4. One porcine isolate was of serotype 0:19, while another isolate from the second Y. enterocolitica-bearing porcine throat swab was nontypeable. The nontypeable isolates autoagglutlnated in tissue culture when grown overnight at 35°C, suggesting that these isolates are potentially virulent. The results of this study support the contention of European researchers that swine may be a significant source of Y. enterocolitica involved in human infections.
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 47 (1982), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Three recently developed tests used to indicate virulence were performed on 34 strains of Yersinia enterocolitica. Agreement in virulence or nonvirulence prediction was found in (a) 29 of 31 strains when tested for calcium growth dependency at 35°C; (b) all 34 strains when tested for the ability to autoagglutinate in tissue culture medium at 35°C; and (c) among all 34 strains when tested for the presence of a 40–48 Mdal plasmid using a modification of the Hansen and Olsen method for plasmid analysis. The autoagglutination assay is recommended for testing potential pathogenicity of food-borne isolates. There are probably several virulence-determining factors for Y. enterocolitica, and no one in-vitro test will suffice. The analytical procedure described herein can be used to detect plasmids ranging in size from approximately 2 × 106 to 3 × 108 daltons.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 46 (1981), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Unwashed carcasses of swine (58), sheep (59), and beef (58) were assessed for the incidence of Campylobacter fetus spp. jejuni. The potential pathogen was present on 38% of the swine, 24% of the sheep, and 2% of the beef carcasses examined. This study serves to point out the association of the pathogen within food marketing channels.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 45 (1980), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effects of pH level and sodium chloride concentration on clinical and environmental strains of Yersinia enterocolitica at 3° and 25°C were determined. Bacteriostatic and bactericidal inhibition was observed at 7% w/v sodium chloride at both 3° and 25° C in Brain Heart Infusion Broth. Growth was observed at pH 4.6-9.0. Clinical strains showed significantly greater tolerance to pH level and sodium chloride concentration than did the environmental strains of Y. enterocolitica at 25° C. These findings suggest that Y. enterocolitica may be resistant to some common methods of food preservation.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 46 (1981), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Pyrolysis gas-liquid chromatography (PGLC) and statistical analysis employing stepwise discriminant analysis (SDA), were used to classify five species and varieties in the genus Bacillus. Nine strains were harvested, lyophilized, and pyrolyzed under replicate conditions. The combination of PGLC and SDA enabled classification of these selected varieties and species of Bacillus at 96% accuracy. Stepwise discriminant analysis of the same PGLC data discriminated (100%) B. cereus, a foodborne pathogen, from the remaining selected nonpathogenic bacilli when the two groups were compared. One particular elution peak resulting from the PGLC-SDA analysis was prominent in both discriminations reported. The PGLC and SDA method offers potential as an accurate, objective procedure for the discrimination of these selected bacilli.
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 46 (1981), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Campylobacter fetus ssp. jejuni has increasingly been implicated as the causative agent in human cases of gastroenteritis. The first account of C. jejuni isolation from red meats—lamb carcasses—is reported. Recovery of Campylobacter from meat surfaces was tested as follows: selective agar plates consisted of tryptic soy agar, 5% lysed defibrinated horse blood, 10 mg/L vancomycin, 2500 units/L polymyxin B, 5 mg/L trimethoprim lactate, and 15 mg/L cephalothin (Keflin; VPTK: plates). These VPTK plates were incubated at 35°C for 72 hr in a gas atmosphere of 5% O2, 10% CO2, and 85% N2. With these selective elements, recovery from meat surfaces inoculated with a calculated 32 Campylobacter cells per cm2 was accomplished in 5 of 5 replicate tests. At levels of 3.2 and 0.3 cells of Campylobacter per cm2, recoveries were accomplished in 2 of 5 replicate tests at each inoculum level.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 45 (1980), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Seventy-three lambs were divided into four treatment groups and fed for 3 months either (1) control diet, (2) control plus 25 ppm of a tertiary amine (N,N-dimethyldodecanamine), (3) control plus 50 ppm of the amine, or (4) control plus 25 ppm chlortetracycline (CTC). The lambs were slaughtered at the end of the feeding trial. Half of the 146 sides were electrically stimulated (ES). Subsequently, half of the ES and half the nonstimulated sides were hot-boned and the other halves were cold-boned at 6 days postmortem. At 6 days postmortem, all racks were vacuum packaged and held at 3°C until 35 days postmortem. Cold- and hot-boned carcasses were processed into lamb chops and packaged in oxygen-permeable wrap and kept under retail market conditions (5°C and 12 hr of lighting/day). Surface swabs were taken from lamb chops initially and after 3 days of retail storage and from lamb racks at 35 days postmortem. The following statistically significant (P 〈 0.05) results were obtained. Standard plate counts (SPCs) and psychrotrophic plate counts (PPCs) were consistently higher for hot-boned lamb chops initially and after 3 days of storage compared with the cold-boned lamb chops initially and after 3 days of storage. The situation was reversed for the lamb racks after 35 days of storage: hot-boned racks had lower counts than the cold-boned racks. PPCs from lamb chops from lambs fed CTC-supplemented diets were lower than PPCs from lamb chops from lambs fed unsupplemented (control) diets after 3 days of storage. Electrical stimulation exerted no significant effect on surface bacterial numbers.
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 45 (1980), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A combination of four Y. enterocolitica strains was inoculated with and without five competing psychrotrophic bacteria, into milk that had been pasteurized using ultra-high-temperature procedures. Milk inoculated at two levels with Y. enterocolitica was held at 3°C and 25°C, without competing bacteria and monitored for growth. At 3°C, cells per ml of milk increased from 250 to more than 4.6 × 107 in less than 3 wk. Growth and identification were determined for Y. enterocolitica and competing organisms in milk held for 10 days at 3°C. After 10 days, the total count was 1.7 × 107 cells per ml of the inoculated milk. Pseudomonas fluorescens accounted for 43% of the 60 randomly picked colonies that were representative of the microbial population in the milk. Competing organisms were isolated in the following order of decreasing frequency: Micrococcus varians, Alcaligenes faecalis, Achromobacter pestifer, and Bacillus cereus. Y. enterocolitica was not present among the colonies randomly picked, but was detected in low numbers in Bismuth Sulfite Agar spread plates. This work indicated that Y. enterocolitica has the capacity for growth in milk at refrigeration temperatures, but is a poor competitor with common spoilage organisms.
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 4 (1982), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Modified Skirrow's (with 15 μg cephalothin/ml), Campy-BAP, and Butzler's selective agar plates were compared for sensitivity and selectivity in the recovery of inoculated Campylobacter fetus ssp. jejuni from ground beef. The diluted inoculation samples were surface plated directly onto these selective plates and incubated. When colonies on the plates were enumerated, characterized, and identified, Campy-BAP medium proved to be the most sensitive for recovery, and Butzler's medium the most selective. Contaminants of Pseudomonas and Acinetobacter were observed. A combination of both Campy-BAP and Butzler's media should prove most useful in the recovery of Campylobacter from food sources. The significance of current taxo-nomical considerations for food microbiologists are also discussed.
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  • 10
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A congenic pair ofCampylobacter jejuni has been previously developed in our laboratory that will (strain A74/C) and will not (strain A74/O) colonize 2-day-old chicks dosed with 105 colony forming units. Outer membrane protein (OMP) extracts of these organisms were prepared and studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analyses. No consistent differences between the colonizer and noncolonizer were detected by SDS-PAGE. However, an antigen of 28 kD molecular weight was consistently found in the noncolonizer, but only at greatly reduced levels or not at all in the colonizer by Western blot analysis with rabbit anti-OMP serum. After affinity purification and cross absorption of serum against OMP from the colonizer, an antigen of 69 kD molecular weight was found unique to the colonizing strain. Exclusive association of the 69 kD antigen with the colonizing strain suggests that it may be a colonization factor.
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