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  • 1
    Publication Date: 1992-11-01
    Print ISSN: 0021-8561
    Electronic ISSN: 1520-5118
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 70 (2005), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : ε–polylysine is a homopolymer of L-lysine, an essential amino acid, with a reportedly wide antimicrobial spectrum. This study evaluated the antimicrobial activity of ε–polylysine, as compared with known preservatives and organic acids, against Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes, in culture broth. The compounds tested included ε–polylysine (0.0025% to 0.05%), sodium diacetate (0.25%), sodium lactate (3.0%), lactic acid (0.1%), and acetic acid (0.1%), alone, as well as in combination with ε– polylysine (0.0025% to 0.03%); all treatments were evaluated in tryptic soy broth supplemented with 0.6% yeast extract. Treatments were inoculated (approximately 2 log colony-forming units [CFU]/mL) with 5-strain (E. coli O157:H7, S. Typhimurium) or 10-strain (L. monocytogenes) mixtures of the pathogens. Survival/growth of the inoculated bacteria was periodically monitored during incubation at 4 °C (30 d) and 24 °C (48 h). Bactericidal effects of ε–polylysine were obtained against E. coli O157:H7 and S. Typhimurium at 4 °C. At the same temperature (4 °C), ε–polylysine alone or in combination with other compounds tested inhibited growth or was bactericidal against L. monocytogenes. All 3 pathogens were inhibited by ε–polylysine at 24 °C; however, L. monocytogenes was the most sensitive and S. Typhimurium the most resistant. The antimicrobial activity of ε–polylysine against E. coli O157:H7 and S. Typhimurium was enhanced (P 〈 0.05) when tested in combination with sodium diacetate or acetic acid. Combination treatments with sodium lactate resulted in loss of ε–polylysine activity by the end of the incubation period. Overall, under the conditions of this study, ε–polylysine exhibited antimicrobial effects against the 3 pathogens tested.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : This study evaluated the influence of drying treatments and aerobic storage (25°C, 30 d) on inactivation of a five-strain mixture of Salmonella (7.8 log colony-forming units [CFU]/g) on carrot slices. Treatments included (1) control, (2) steam blanching (88°C, 3 min), (3) water blanching (88°C, 3 min), (4) immersion in 3.23% NaCl (25 ± 3°C, 5 min), and (5) oven heating (80°C, 15 min) after drying. Treatments were selected from recommendations made by Cooperative Extension Services for ability to maintain characteristics of dried vegetables and possible antimicrobial effects. Carrot slices were inoculated with the Salmonella mixture, left for 15 min to allow for attachment, then treated (steam blanched, water blanched, or 3.23% NaCl immersion) and dehydrated (60°C, 6 h), or left untreated, dehydrated (60°C, 6 h), and heated (80°C, 15 min). Samples were analyzed by spread-plating on tryptic soy agar with 0.1% pyruvate (TSAP) and xylose lysine deoxycholate (XLD) agar for bacterial enumeration. Initial populations (6.96 to 7.18 log CFU/g) were reduced by 3.2 to 3.3 log CFU/g immediately after steam or water blanching, and by 0.6 log CFU/g following immersion in 3.23% NaCl. After 6 h dehydration, reductions were 1.3 to 2.0 (control), 4.0 to 4.7 (steam blanched), 3.5 to 4.3 (water blanched), and 1.9 to 2.6 (3.23% NaCl) log CFU/g. Reductions on samples heated after drying were 1.7 to 2.4 log CFU/g. All samples had populations 〉1.7 log CFU/g after 6 h drying and 30 d storage at 25°C and, therefore, may pose a food safety risk. Modified treatments are needed to further enhance inactivation of Salmonella on dehydrated carrots.
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  • 4
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Beef slices were inoculated (5.7–7.5 log CFU/cm2) with a 4-strain composite of E. coli O157:H7, stored (4C, 24 h), marinated (4C, 24 h), dried for 10 h at 62.5C or 68.3C, and stored for 90 days at 21C. Unmarinated beef slices dried for 10 h at 62.5C were used to determine the relative contribution of the marinate versus temperature treatment in the 62.5C trials. Samples were analyzed (bacterial enumeration with selective and nonselective agar media, pH, and aw) following inoculation, marinating, at 4, 6, 8 and 10 h of drying, and after 30, 60 and 90 days of storage. Marination resulted in slight changes in bacterial populations (−0.3 to + 0.6 log CFU/cm2), but did not enhance bacterial reduction during drying. For all treatments, most bacterial reductions occurred in the first 4 h of drying, with little reduction thereafter. After 10 h of drying, bacterial reductions were 3.2–3.4 log CFU/cm2 for unmarinated beef slices dried at 62.5C. Reductions of 2.2 and 3.0–4.6 log CFU/cm2 were achieved in marinated jerky slices dried at 62.5C and 68.3C, respectively. No treatment resulted in the recommended 5-log reduction at the end of 10 h drying. However, bacteria did become undetectable by direct plating (〈10 CFU/cm2) following 30 days of storage in all treatments except the unmarinated beef slices plated on tryptic soy agar (TSA). Additional work is needed to develop procedures for adequate destruction of E. coli O157:H7 during drying of beef jerky.
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  • 5
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 40 (1992), S. 2182-2185 
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 59 (1994), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Sliced cooked turkey bologna with various additive formulations was surface-inoculated with Listeria monocytogenes (2.06–2.75 log CFU/g), vacuum packaged, and stored at 4°C. Sodium acetate was most inhibitory against growth of L. monocytogenes, followed by sodium lactate and potassium sorbate, while sodium bicarbonate allowed a maximum net growth of 6.78 log CFU/g, not significantly different (p〉0.05) from the control (6.43 log CFU/g). Addition of 0.5% sodium acetate, 2.0% sodium lactate, or 0.26% potassium sorbate may significantly (p〈0.05) decrease growth of L. monocytogenes in refrigerated turkey bologna surface-inoculated after thermal processing and slicing.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 51 (1986), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Uncured turkey and chicken breast meat products, formulated with NaCl (0.65 and 1.30%) and potassium sorbate (0 and 0.26%), were tested for antimicrobial activity and functional properties. The chopped products were inoculated (Clostridium sporogenes, 10/g) in cans and in packages and abused at 20°C. Microbial growth and gas production were rapid at both NaCl levels tested. Inclusion of potassium sorbate in the formulation delayed initiation and rate of microbial growth and gas production. Potassium sorbate also reduced (P〈0.05) weight losses during cooking of the low NaCl turkey product. Rates of gas production were similar in canned and vacuum packaged products. Differences in microbial growth and gas production between chicken and turkey products were minor.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 50 (1985), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of varying NaCl levels (0.8, 1.2, 1.6, 2.0, and 2.4%) on the cooking yield and composition of comminuted meat (beef-pork) products (frankfurters and cans) was tested in the absence and presence of 0.26% potassium sorbate. Parameters measured included frankfurter smokehouse yields and consumer cook losses; product losses in cans; and, moisture and fat contents of the cooked products. Reducing the NaCl level resulted in increased product losses during cooking. Potassium sorbate decreased these losses. Cooking yields at most NaCl levels (≦ 1.6%) tested were improved (P〈0.01) with the addition of potassium sorbate. The positive influence of sorbate on product binding could not be attributed to pH or ionic strength changes, but potential mechanisms of sorbate action are discussed.
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 50 (1985), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The binding and antimicrobial properties of comminuted meat products were evaluated at varying levels of NaCl and of sodium tripolyphosphate (STPP). The meat batters were inoculated with Clostridium sporogenes spores (10/g) either before (cans) or after (vacuum packages) thermal (70°C) processing. Product binding was inferior when the NaCl level was reduced to 1.1% (50% reduction). STPP increased pH by 0.17-0.23 units and restored binding of low NaCl products. During storage (20°C) microbial growth and product spoilage were more rapid with decreasing brine level, irrespective of presence or absence of STPP.
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 56 (1991), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Differential scanning calorimetry thermal curves of beef semimembranosus muscle showed peaks at 58, 67 and 78°C. Addition of algin/calcium binder or 5-10% connective tissue increased magnitude of the 58°C peak. For crude myofibrillar, sarcoplasmic and connective tissue proteins thermal destabilization was lowered by 7.5, 23.6 and 8.6°C, respectively, with addition of algin/calcium binder. Destabilization of tendon by the binder from about 67°C to 58°C was similar in magnitude to the shift of the 67-68 and 58°C thermal transitions reported in semimembranosus and probably indicated collagen destabilization. Thermal destabilization by algin/calcium may indicate change in physical state of proteins which could influence texture of algin/calcium restructured meat products.
    Type of Medium: Electronic Resource
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