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  • 1
    Publication Date: 2011-05-25
    Description: Rapid induction of CD8+ cytotoxic T lymphocyte (CTL) responses is critical to combat acute infection with intracellular pathogens. CD4+ T cells help prime antigen-specific CTLs in secondary lymphoid organs after infection in the periphery. Although the frequency of naïve precursors is very low, the immune system is able to efficiently screen for cognate CTLs through mechanisms that are not well understood. Here we examine the role of CD4+ T cells in early phases of the immune response. We show that CD4+ T cells help optimal CTL expansion by facilitating entry of naïve polyclonal CD8+ T cells into the draining lymph node (dLN) early after infection or immunization. CD4+ T cells also facilitate input of naïve B cells into reactive LNs. Such “help” involves expansion of the arteriole feeding the dLN and enlargement of the dLN through activation of dendritic cells. In an antigen- and CD40-dependent manner, CD4+ T cells activate dendritic cells to support naïve lymphocyte recruitment to the dLN. Our results reveal a previously unappreciated mode of CD4+ T-cell help, whereby they increase the input of naïve lymphocytes to the relevant LN for efficient screening of cognate CD8+ T cells.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 2
    Publication Date: 2014-05-03
    Description: Coupled U-Pb and Hf isotopic analysis of detrital zircons from metasedimentary rocks of the Southern Granulite terrane (India) provides provenance information that helps unravel their paleotectonic position before Gondwana amalgamated. The metasedimentary packages of the Salem block (southernmost extension of Dharwar craton) record a restricted juvenile late Archean to early Paleoproterozoic (2.7–2.45 Ga) source provenance and epsilon Hf values between +0.3 and +8.8. Similar late Archean juvenile crust is found throughout the Dharwar craton and represents a likely source for the Salem block metasedimentary rocks. By contrast, the metasedimentary rocks of the Madurai block (south of the Salem block) show a predominantly Archean to Paleoproterozoic provenance (3.2–1.7 Ga) in the northern part of the Madurai block and a largely late Mesoproterozoic to Neoproterozoic provenance (1.1–0.65 Ga) in the southern part of the Madurai block. Collectively, the Madurai block metasedimentary rocks record a mixture of reworked Archean and Paleoproterozoic sources, as well as juvenile Paleoproterozoic, late Mesoproterozoic, and evolved Neoproterozoic sources. These detrital signatures best fit the combined basement ages of the Congo-Tanzania-Bangweulu block and central Madagascar (Azania), thus linking the tectonic evolution of the southernmost tip of India to these domains throughout much of the Proterozoic. The diachroneity of metamorphic ages obtained from the rims of Madurai block detrital zircons attests to their poly-metamorphic history that is different from that of the Salem block. The contrasting metamorphic and depositional histories between the Salem and Madurai blocks place them on opposite sides of the Mozambique Ocean until the latest Neoproterozoic when they came together to form Gondwana.
    Print ISSN: 0016-7606
    Electronic ISSN: 1943-2674
    Topics: Geosciences
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  • 3
    Publication Date: 2008-08-12
    Description: The domestication of cattle, sheep and goats had already taken place in the Near East by the eighth millennium bc. Although there would have been considerable economic and nutritional gains from using these animals for their milk and other products from living animals-that is, traction and wool-the first clear evidence for these appears much later, from the late fifth and fourth millennia bc. Hence, the timing and region in which milking was first practised remain unknown. Organic residues preserved in archaeological pottery have provided direct evidence for the use of milk in the fourth millennium in Britain, and in the sixth millennium in eastern Europe, based on the delta(13)C values of the major fatty acids of milk fat. Here we apply this approach to more than 2,200 pottery vessels from sites in the Near East and southeastern Europe dating from the fifth to the seventh millennia bc. We show that milk was in use by the seventh millennium; this is the earliest direct evidence to date. Milking was particularly important in northwestern Anatolia, pointing to regional differences linked with conditions more favourable to cattle compared to other regions, where sheep and goats were relatively common and milk use less important. The latter is supported by correlations between the fat type and animal bone evidence.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Evershed, Richard P -- Payne, Sebastian -- Sherratt, Andrew G -- Copley, Mark S -- Coolidge, Jennifer -- Urem-Kotsu, Duska -- Kotsakis, Kostas -- Ozdogan, Mehmet -- Ozdogan, Asly E -- Nieuwenhuyse, Olivier -- Akkermans, Peter M M G -- Bailey, Douglass -- Andeescu, Radian-Romus -- Campbell, Stuart -- Farid, Shahina -- Hodder, Ian -- Yalman, Nurcan -- Ozbasaran, Mihriban -- Bicakci, Erhan -- Garfinkel, Yossef -- Levy, Thomas -- Burton, Margie M -- England -- Nature. 2008 Sep 25;455(7212):528-31. doi: 10.1038/nature07180. Epub 2008 Aug 6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Organic Geochemistry Unit, Bristol Biogeochemistry Research Centre, School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS, UK. r.p.evershed@bristol.ac.uk〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18690215" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cattle/*physiology ; Ceramics/analysis ; Dairying/*history ; Europe ; Europe, Eastern ; Fatty Acids/analysis ; Goats ; History, Ancient ; Humans ; Meat ; Milk/chemistry/*history/*utilization ; Sheep
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
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  • 4
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 1989-09-22
    Description: Yeast mutants deficient in the clathrin heavy chain secrete a precursor form of the alpha-factor, a peptide-mating pheromone. Analysis of this defect indicates that the endoprotease Kex2p, which is responsible for initiating proteolytic maturation of the alpha-factor precursor in the Golgi apparatus, is unexpectedly present at the plasma membrane in mutant cells. This result suggest that clathrin is required for the retention of Kex2p in the Golgi apparatus.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Payne, G S -- Schekman, R -- GM 36881/GM/NIGMS NIH HHS/ -- GM 39040/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1989 Sep 22;245(4924):1358-65.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biological Chemistry, UCLA School of Medicine 90024.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2675311" target="_blank"〉PubMed〈/a〉
    Keywords: Cell Compartmentation ; Clathrin/*physiology ; Golgi Apparatus/*physiology ; Intracellular Membranes/*physiology ; Membrane Proteins/*physiology ; Peptide Hydrolases/metabolism ; Peptides/metabolism ; Protein Precursors/metabolism ; Protein Processing, Post-Translational ; Saccharomyces cerevisiae
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 5
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 1985-11-29
    Description: Clathrin-coated membranes are intimately associated with a variety of protein transport processes in eukaryotic cells, yet no direct test of clathrin function has been possible. The data presented demonstrate that Saccharomyces cerevisiae does not require clathrin for either cell growth or protein secretion. Antiserum to the yeast clathrin heavy chain has been used to isolate a molecular clone of the heavy chain gene (CHC1) from a library of yeast DNA in lambda gt11. Clathrin-deficient mutant yeast have been obtained by replacing the single chromosomal CHC1 gene with a disrupted version of the cloned DNA. Cells harboring a nonfunctional chc1 allele produce no immunoreactive heavy chain polypeptide, and vesicles prepared from mutant cells are devoid of clathrin heavy and light chains. Although clathrin-deficient cells grow two to three times more slowly than normal, secretion of invertase occurs at a nearly normal rate. Therefore protein transport through the secretory pathway is not obligately coupled to the formation of clathrin-coated vesicles.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Payne, G S -- Schekman, R -- New York, N.Y. -- Science. 1985 Nov 29;230(4729):1009-14.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2865811" target="_blank"〉PubMed〈/a〉
    Keywords: Biological Transport ; *Cell Physiological Phenomena ; Clathrin/genetics/immunology/*physiology ; Coated Pits, Cell-Membrane/*physiology ; Endosomes/*physiology ; Eukaryotic Cells/*physiology ; Genes ; Genes, Fungal ; Genetic Engineering ; Glycoside Hydrolases/secretion ; Macromolecular Substances ; Molecular Weight ; Proteins/*secretion ; Saccharomyces cerevisiae/genetics ; beta-Fructofuranosidase
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 6
    Publication Date: 2018-08-02
    Description: Evolution of high pathogenicity of H5 avian influenza virus: haemagglutinin cleavage site selection of reverse-genetics mutants during passage in chickens Evolution of high pathogenicity of H5 avian influenza virus: haemagglutinin cleavage site selection of reverse-genetics mutants during passage in chickens, Published online: 01 August 2018; doi:10.1038/s41598-018-29944-z Evolution of high pathogenicity of H5 avian influenza virus: haemagglutinin cleavage site selection of reverse-genetics mutants during passage in chickens
    Electronic ISSN: 2045-2322
    Topics: Natural Sciences in General
    Published by Springer Nature
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  • 7
    Publication Date: 2014-06-21
    Description: Article Pemphingus vulgaris is known to be caused by anti-desmoglein 3 autoantibodies, but how these antibodies arise is not clear. Here, the authors show that VH gene usage is shared among patients and that few or no somatic mutations are required for these autoantibodies to acquire autoreactivity. Nature Communications doi: 10.1038/ncomms5167 Authors: Michael Jeffrey Cho, Agnes S.Y. Lo, Xuming Mao, Arielle R. Nagler, Christoph T. Ellebrecht, Eric M. Mukherjee, Christoph M. Hammers, Eun-Jung Choi, Preety M. Sharma, Mohamed Uduman, Hong Li, Ann H. Rux, Sara A. Farber, Courtney B. Rubin, Steven H. Kleinstein, Bruce S. Sachais, Marshall R. Posner, Lisa A. Cavacini, Aimee S. Payne
    Electronic ISSN: 2041-1723
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General , Physics
    Published by Springer Nature
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  • 8
    Publication Date: 2013-11-09
    Description: Penicillium marneffei is an opportunistic human pathogen endemic to Southeast Asia. At 25° P. marneffei grows in a filamentous hyphal form and can undergo asexual development (conidiation) to produce spores (conidia), the infectious agent. At 37° P. marneffei grows in the pathogenic yeast cell form that replicates by fission. Switching between these growth forms, known as dimorphic switching, is dependent on temperature. To understand the process of dimorphic switching and the physiological capacity of the different cell types, two microarray-based profiling experiments covering approximately 42% of the genome were performed. The first experiment compared cells from the hyphal, yeast, and conidiation phases to identify "phase or cell-state–specific" gene expression. The second experiment examined gene expression during the dimorphic switch from one morphological state to another. The data identified a variety of differentially expressed genes that have been organized into metabolic clusters based on predicted function and expression patterns. In particular, C-14 sterol reductase–encoding gene ergM of the ergosterol biosynthesis pathway showed high-level expression throughout yeast morphogenesis compared to hyphal. Deletion of ergM resulted in severe growth defects with increased sensitivity to azole-type antifungal agents but not amphotericin B. The data defined gene classes based on spatio-temporal expression such as those expressed early in the dimorphic switch but not in the terminal cell types and those expressed late. Such classifications have been helpful in linking a given gene of interest to its expression pattern throughout the P. marneffei dimorphic life cycle and its likely role in pathogenicity.
    Electronic ISSN: 2160-1836
    Topics: Biology
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  • 9
    Publication Date: 2017-03-29
    Description: Phosphoinositides serve as key membrane determinants for assembly of clathrin coat proteins that drive formation of clathrin-coated vesicles. At the trans-Golgi network (TGN), phosphatidylinositol 4-phosphate (PtdIns4P) plays important roles in recruitment of two major clathrin adaptors, Gga (Golgi-localized, gamma-adaptin ear homology, Arf-binding) proteins and the AP-1 (assembly protein-1) complex. The...
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 10
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Materials Research 23 (1993), S. 453-502 
    ISSN: 0084-6600
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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